Three Y-Chromosome STR Frequencies in a Population from Equatorial Guinea (Central Africa)

Specimens were collected from unrelated apparently healthy males, blood donors from Equatorial Guinea (Central Africa). DNA was extracted from blood specimens using a bloodclean DNA purification Kit (Biotools) and typed in an ALF-Sequencer (Pharmacia). DYS19 alleles were determined according to prot...

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Bibliographic Details
Published in:Journal of forensic sciences 2002-01, Vol.47 (1), p.224-225
Main Authors: Alvarez, S, López-Parra, AM, Mesa, MS, Jiménez, V, Herrera, SJ, Bandrés, F, Arraztio, A, Rubio, JM, Arroyo-Pardo, E
Format: Article
Language:English
Subjects:
DNA Fingerprinting
Forensic Medicine - methods
Gene Frequency
Guinea
Humans
Male
Polymerase Chain Reaction
Reference Values
Tandem Repeat Sequences - genetics
Y Chromosome - genetics
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Summary:Specimens were collected from unrelated apparently healthy males, blood donors from Equatorial Guinea (Central Africa). DNA was extracted from blood specimens using a bloodclean DNA purification Kit (Biotools) and typed in an ALF-Sequencer (Pharmacia). DYS19 alleles were determined according to protocols and allelic ladders kindly supplied by Peter de Knijff (1). Primers for DYS389I and DYS389II were synthesized according to Schultes et al. (2). PCR conditions for these two systems were modified as follows: a first denaturation step at 94°C 3 min; 5 cycles of 94°C 15 s, 58#x00B0;C 20 s, 72#x00B0;C 20 s, 34 cycles of 94#x00B0;C 15 s, 54#x00B0;C 20 s, 72#x00B0;C 20 s. Amplification products were typed with allelic ladders from our laboratory. Frequencies were calculated for all the systems through the gene counting method and gene diversity was estimated according to Nei (3).
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS15232J