Genetic Variation at Three DYS-STR Loci and Amelogenin Between the Australian Caucasians and Three Asian Ethnic Groups

Blood samples were obtained from unrelated male individuals from the four population groups and placed on FTA® paper (Fitzco-Whatman). DNA was extracted using the aqueous processing method for FTA® paper. One extracted disc (1.2 mm diameter) was used in the PCR per sample. The mixed male DNA sample...

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Bibliographic Details
Published in:Journal of forensic sciences 2002-07, Vol.47 (4), p.1-4
Main Authors: Chang, YM, Both, K, Burgoyne, LA
Format: Article
Language:English
Subjects:
Asia - ethnology
Australia
Ethnic Groups
European Continental Ancestry Group - genetics
Forensic Medicine - methods
Forensic Medicine - statistics & numerical data
Genetic Variation
Humans
Polymerase Chain Reaction
Reference Values
Tandem Repeat Sequences - genetics
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Summary:Blood samples were obtained from unrelated male individuals from the four population groups and placed on FTA® paper (Fitzco-Whatman). DNA was extracted using the aqueous processing method for FTA® paper. One extracted disc (1.2 mm diameter) was used in the PCR per sample. The mixed male DNA sample was loaded onto a pre-washed FTA® paper and subjected to an alkalineisopropanol treatment. A combination of three Y-linked microsatellites loci, DYS438 (1), DYS390 (2), and DYS439 (1) and amelogenin were amplified simultaneously using a fluorescent multiplex system. DYS438 and amelogenin were labelled with 6-FAM, DYS390 with JOE and DYS439 with TAMRA.
ISSN:0022-1198
DOI:10.1520/JFS15483J