The DRPLA CAG Repeats in an Italian Population Sample: Evaluation of the Polymorphism for Forensic Applications

The DRPLA CAG repeats polymorphism has been studied in an Italian population sample. PCR amplification, manual PAGE and silver staining were employed. A total of 16 different alleles, spanning the range from 5 to 21 CAG triplettes, was observed. The heterozygosity was 0.81 and no significant deviati...

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Bibliographic Details
Published in:Journal of forensic sciences 1998-03, Vol.43 (2), p.410-412
Main Authors: Pelotti, S, Mantovani, V, Esposti, PD, D'Apote, L, Bragliani, M, Maiolini, E, Abbondanza, A, Pappalardo, G
Format: Article
Language:English
Subjects:
Alleles
DNA - analysis
DNA Fingerprinting - methods
DNA Primers - chemistry
Female
Humans
Italy
Male
Nerve Tissue Proteins - genetics
Neurodegenerative Diseases - genetics
Polymerase Chain Reaction
Polymorphism, Genetic
Trinucleotide Repeats - genetics
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Summary:The DRPLA CAG repeats polymorphism has been studied in an Italian population sample. PCR amplification, manual PAGE and silver staining were employed. A total of 16 different alleles, spanning the range from 5 to 21 CAG triplettes, was observed. The heterozygosity was 0.81 and no significant deviation from Hardy-Weinberg equilibrium was found. 81 meioses from parentage testing were also analyzed and a Mendelian pattern of inheritance was observed in all cases. In addition, we could successfully type DRPLA locus in some forensic specimens, 1 ng of DNA allowing clear definition of alleles. The authors conclude that the DRPLA CAG repeats analysis may be useful for forensic applications.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS16158J