Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut

A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified...

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Bibliographic Details
Published in:Electronic Journal of Biotechnology 2011-04, Vol.13 (5)
Main Authors: Wang, Chuan Tang, Yu, Shan Lin, Zhang, Shu Wei, Wang, Xiu Zhen, Tang, Yue Yi, Zhang, Jian Cheng, Chen, Dian Xu
Format: Article
Language:English
Subjects:
identification, NIRS, PCR, peanut, true hybrid
Online Access:Get full text
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Summary:A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.
ISSN:0717-3458
0717-3458