Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey

A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae . Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (B...

Full description

Saved in:
Bibliographic Details
Published in:Electronic Journal of Biotechnology 2011-04, Vol.13 (6)
Main Authors: Erisen, Semiha, Yorgancilar, Mustafa, Atalay, Emine, Babaoglu, Mehmet, Duran, Ahmet
Format: Article
Language:English
Subjects:
astragalus, callus, organogenesis, plant regeneration
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page
container_issue 6
container_start_page
container_title Electronic Journal of Biotechnology
container_volume 13
creator Erisen, Semiha
Yorgancilar, Mustafa
Atalay, Emine
Babaoglu, Mehmet
Duran, Ahmet
description A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae . Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.
format article
fullrecord <record><control><sourceid>bioline</sourceid><recordid>TN_cdi_bioline_primary_cria_bioline_ej_ej10079</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>cria_bioline_ej_ej10079</sourcerecordid><originalsourceid>FETCH-bioline_primary_cria_bioline_ej_ej100793</originalsourceid><addsrcrecordid>eNqVjM0KgkAUhYcoyH7e4b6AMaYxtQwpegBby02vNjqOMjMu7OmzKGgbHDiHD74zYR4XgfDDaLef_uw5W1hbcb7lkYg8do1Rqd6C1HmfOdlqQJ1Dp1A7MFSSJoNv3Bbg7gSkc2pkBkfrDJb4UjU9sCaHNJ5A0puahhWbFagsrT-9ZJvzKYkv_k22SmpKOyMbNEOaGYnpF1I1JuBcHMK_hSegBkzW</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey</title><source>SciELO</source><creator>Erisen, Semiha ; Yorgancilar, Mustafa ; Atalay, Emine ; Babaoglu, Mehmet ; Duran, Ahmet</creator><creatorcontrib>Erisen, Semiha ; Yorgancilar, Mustafa ; Atalay, Emine ; Babaoglu, Mehmet ; Duran, Ahmet</creatorcontrib><description>A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae . Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.</description><identifier>ISSN: 0717-3458</identifier><identifier>EISSN: 0717-3458</identifier><language>eng</language><publisher>Universidad Católica de Valparaíso</publisher><subject>astragalus, callus, organogenesis, plant regeneration</subject><ispartof>Electronic Journal of Biotechnology, 2011-04, Vol.13 (6)</ispartof><rights>Copyright 2010 - Pontificia Universidad Católica de Valparaíso -- Chile</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781</link.rule.ids></links><search><creatorcontrib>Erisen, Semiha</creatorcontrib><creatorcontrib>Yorgancilar, Mustafa</creatorcontrib><creatorcontrib>Atalay, Emine</creatorcontrib><creatorcontrib>Babaoglu, Mehmet</creatorcontrib><creatorcontrib>Duran, Ahmet</creatorcontrib><title>Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey</title><title>Electronic Journal of Biotechnology</title><description>A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae . Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.</description><subject>astragalus, callus, organogenesis, plant regeneration</subject><issn>0717-3458</issn><issn>0717-3458</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqVjM0KgkAUhYcoyH7e4b6AMaYxtQwpegBby02vNjqOMjMu7OmzKGgbHDiHD74zYR4XgfDDaLef_uw5W1hbcb7lkYg8do1Rqd6C1HmfOdlqQJ1Dp1A7MFSSJoNv3Bbg7gSkc2pkBkfrDJb4UjU9sCaHNJ5A0puahhWbFagsrT-9ZJvzKYkv_k22SmpKOyMbNEOaGYnpF1I1JuBcHMK_hSegBkzW</recordid><startdate>20110411</startdate><enddate>20110411</enddate><creator>Erisen, Semiha</creator><creator>Yorgancilar, Mustafa</creator><creator>Atalay, Emine</creator><creator>Babaoglu, Mehmet</creator><creator>Duran, Ahmet</creator><general>Universidad Católica de Valparaíso</general><scope>RBI</scope></search><sort><creationdate>20110411</creationdate><title>Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey</title><author>Erisen, Semiha ; Yorgancilar, Mustafa ; Atalay, Emine ; Babaoglu, Mehmet ; Duran, Ahmet</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-bioline_primary_cria_bioline_ej_ej100793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>astragalus, callus, organogenesis, plant regeneration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Erisen, Semiha</creatorcontrib><creatorcontrib>Yorgancilar, Mustafa</creatorcontrib><creatorcontrib>Atalay, Emine</creatorcontrib><creatorcontrib>Babaoglu, Mehmet</creatorcontrib><creatorcontrib>Duran, Ahmet</creatorcontrib><collection>Bioline International</collection><jtitle>Electronic Journal of Biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Erisen, Semiha</au><au>Yorgancilar, Mustafa</au><au>Atalay, Emine</au><au>Babaoglu, Mehmet</au><au>Duran, Ahmet</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey</atitle><jtitle>Electronic Journal of Biotechnology</jtitle><date>2011-04-11</date><risdate>2011</risdate><volume>13</volume><issue>6</issue><issn>0717-3458</issn><eissn>0717-3458</eissn><abstract>A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae . Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.</abstract><pub>Universidad Católica de Valparaíso</pub></addata></record>
fulltext fulltext
identifier ISSN: 0717-3458
ispartof Electronic Journal of Biotechnology, 2011-04, Vol.13 (6)
issn 0717-3458
0717-3458
language eng
recordid cdi_bioline_primary_cria_bioline_ej_ej10079
source SciELO
subjects astragalus, callus, organogenesis, plant regeneration
title Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T19%3A15%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-bioline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Callus%20induction%20and%20plant%20regeneration%20of%20the%20endemic%20Astragalus%20nezaketae%20in%20Turkey&rft.jtitle=Electronic%20Journal%20of%20Biotechnology&rft.au=Erisen,%20Semiha&rft.date=2011-04-11&rft.volume=13&rft.issue=6&rft.issn=0717-3458&rft.eissn=0717-3458&rft_id=info:doi/&rft_dat=%3Cbioline%3Ecria_bioline_ej_ej10079%3C/bioline%3E%3Cgrp_id%3Ecdi_FETCH-bioline_primary_cria_bioline_ej_ej100793%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true