Identification of chilling-responsive transcripts in peanut ( Arachis hypogaea L.)

To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2°C and 15°C, respectively, for 24 hrs. A total of 466 cDNA cl...

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Published in:Electronic Journal of Biotechnology 2012-10, Vol.14 (5)
Main Authors: Tang, Yue Yi, Wang, Chuan Tang, Yang, Guan Pin, Feng, Tong, Gao, Hua Yuan, Wang, Xiu Zhen, Chi, Xiao Yuan, Xu, Ya Long, Wu, Qi, Chen, Dian Xu
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Language:English
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chilling response, peanut, real-time quantitative PCR, suppression subtractive hybridization
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container_title Electronic Journal of Biotechnology
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creator Tang, Yue Yi
Wang, Chuan Tang
Yang, Guan Pin
Feng, Tong
Gao, Hua Yuan
Wang, Xiu Zhen
Chi, Xiao Yuan
Xu, Ya Long
Wu, Qi
Chen, Dian Xu
description To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2°C and 15°C, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).
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subjects chilling response, peanut, real-time quantitative PCR, suppression subtractive hybridization
title Identification of chilling-responsive transcripts in peanut ( Arachis hypogaea L.)
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