Callus induction and plant regeneration of Ulex europaeus

Abstract A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus . Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best fr...

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Bibliographic Details
Published in:Electronic Journal of Biotechnology 2014-07, Vol.15 (4)
Main Authors: Ramírez, Ingrid, Dorta, Fernando, Cuadros-Inostroza, Álvaro, Peña-Cortés, Hugo
Format: Article
Language:English
Subjects:
antioxidants
callus induction
Fabaceae
gorse
plant regeneration
Ulex europaeus
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Summary:Abstract A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus . Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite.
ISSN:0717-3458
0717-3458