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Biofilm forming capacity and antibiotic susceptibility of Staphylococcus spp. with the icaA/icaD/bap genotype isolated from ocular surface of patients with diabetes
IntroductionBacterial biofilm is an exopolysaccharide matrix that is produced by bacteria while they adhere on abiotic or biotic surfaces. The bacteria living in this matrix are more resistant to antibiotics than planctonic bacteria. The biofilm formation property of the bacteria is determined by ge...
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Published in: | Malawi medical journal 2019-05, Vol.30 (4) |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | IntroductionBacterial biofilm is an exopolysaccharide matrix that is
produced by bacteria while they adhere on abiotic or biotic surfaces.
The bacteria living in this matrix are more resistant to antibiotics
than planctonic bacteria. The biofilm formation property of the
bacteria is determined by genes; and this is related to virulence of
the microorganism. In ophthalmology, biofilms form especially on
abiotic surfaces such as silicon tubes, contact lenses, intraocular
lenses etc. Aim Our aim was to investigate genotypic and phenotypic
structures of biofilms that are produced by Staphylococcus spp., which
was obtained from the eyes of diabetic patients and determine the
effect on antibiotic susceptibility. MethodsThe study group was
comprised with 83 isolates from diabetic patients and 21 isolates from
non-diabetic patients. Presumptive isolates were detected and confirmed
by a microbial identification system VITEK II. Automated EcoRI
Ribotyping was performed. Biofilm production was detected by Congo Red
Agar Plate and Microtiter Plate Assay. Disc diffusion method was used
for determination of antibiotic susceptibility of isolates. ResultsOut
of the 83 isolates from diabetic patients, 25 were weakly (30%), 20
were moderately (24%), and 25 were strongly (30%) biofilm positive.
Seven isolates of S. aureus , 11 isolates of S.epidermidis , 2
isolates of S. warneri , 3 isolates of S.hominis , and 2 isolates of
S.lugdunensis were identified as strong biofilm producers. Out of the
83 Staphylococcus isolates, 37 were cefuroxime, 18 ciprofloxacin, 11
vancomycin, 12 gatifloxacin, and 18 moxifloxacin resistant. In total,
37 strains were resistant to three or more antibiotics. There was a
statistically significant relation between biofilm formation and
multidrug resistance (against three or more antibiotics,p |
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ISSN: | 1995-7262 |