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Preliminary Isolation and in vitro Antiyeast Activity of Active Fraction from Crude Extract of Gracilaria changii

Objective: To isolate the active fraction from crude extract of Gracilaria changii and to determine its in vitro antifungal activity. Materials and Methods: The active fraction was isolated from the crude extract of G. changii by various purification procedures such as column chromatography, thin la...

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Bibliographic Details
Published in:Indian journal of pharmacology 2009-10, Vol.40 (5)
Main Authors: Sasidharan, Sreenivasan, Darah, Ibrahim, Noordin Mohd Kassim, Mohd Jain
Format: Article
Language:English
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Summary:Objective: To isolate the active fraction from crude extract of Gracilaria changii and to determine its in vitro antifungal activity. Materials and Methods: The active fraction was isolated from the crude extract of G. changii by various purification procedures such as column chromatography, thin layer chromatography, bioauthograph etc. The in vitro antifungal activity ( Candida albicans ) of the active fraction (1.00, 0.50, and 0.25 mg/ml) was studied by disc diffusion method and the effect of the active fraction on the morphology of yeast was done by scanning electron microscope (SEM) studies. Results: An active fraction with remarkable antifungal activity was separated from the crude extract. The active fraction was effective as a fungicide against C. albicans and showed a dose-dependent antifungal activity. A Scanning Electron Microscope (SEM) study confirmed the fungicidal effect of G. changii active fraction on C. albicans, by changing the normal morphology of C. albicans. Conclusion: From G. changii crude extract, an active fraction with remarkable in vitro antifungal activity has been isolated.
ISSN:0253-7613