PROTECTIVE EFFECT OF A MEXICAN PROPOLIS ON MDBK CELLS EXPOSED TO AUJESZKY\'S DISEASE VIRUS (PSEUDORABIES VIRUS)

Background: In this paper, the evaluation of the antiviral effect of a Mexican propolis on Pseudo Rabies Virus (PRV) was performed by infecting cell cultures of MDBK. Materials and Methods: First, the level of cytotoxicity of the ethanol extract of propolis (EEP) was determined, subsequently, infect...

Full description

Saved in:
Bibliographic Details
Published in:African journal of traditional, complementary, and alternative medicines complementary, and alternative medicines, 2015-07, Vol.12 (4)
Main Authors: Búrquez, María de Jesús González, Mosqueda, María de Lourdes Juárez, Mendoza, Humberto Ramírez, Zárate, Carlos I. Soto, Miranda, Liborio Carrillo, Sánchez, Tonatiuh Alejandro Cruz
Format: Article
Language:English
Subjects:
antiviral activity
Aujeszky's disease virus
Mexican propolis
Pseudo Rabies Virus (PRV)
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: In this paper, the evaluation of the antiviral effect of a Mexican propolis on Pseudo Rabies Virus (PRV) was performed by infecting cell cultures of MDBK. Materials and Methods: First, the level of cytotoxicity of the ethanol extract of propolis (EEP) was determined, subsequently, infective dose of PRV strain Shope was determined and finally, we performed the interaction of the virus with EEP (two hours before, during and two hours after infection) in MDBK cells. Also, in order to determine the possible effects of propolis on the virus, cell culture samples subjected to the different treatments mentioned above were processed for Transmission Electron Microscopy (TEM). Also, cell culture samples with the same treatments were processed for Transmission Electron Microscopy (TEM) to observe possible alterations of propolis on the virus. Results: It was determined that administration of 0.5 mg / ml of EEP two hours before infection caused a reduction in the number of plaque forming units compared to the rest of the treatments used or with the infected and untreated culture, it is important to note that the difference found was statistically significant (p
ISSN:0189-6016