Study on the binding mode of Mg（Sal2trien） with DNA

In this study the complex Mg（Sal2trien） was synthesized for the first time, the binding mode of which with CT DNA was studied by the methods of UV spectra, fluorescence spectra, viscosity and CV （cyclic voltammetry）. It was found that after the complex acted with CT DNA, the Abs of UV spectra rose o...

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Bibliographic Details
Published in:Chinese science bulletin 2006, Vol.51 (19), p.2322-2326
Main Author: XI Xiaoli YANG Manman ZHOU Chengyong ZHAO Jing YANG Pin
Format: Article
Language:English
Subjects:
三亚乙基四胺
循环伏安法
牛胸腺DNA
荧光光谱
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Summary:In this study the complex Mg（Sal2trien） was synthesized for the first time, the binding mode of which with CT DNA was studied by the methods of UV spectra, fluorescence spectra, viscosity and CV （cyclic voltammetry）. It was found that after the complex acted with CT DNA, the Abs of UV spectra rose obviously; the fluorescence intensity of EB-DNA was almost not changed; viscosity decreased. Determination of cyclic voltammetry showed that DNA made the MgL＇s formal potential negatively shift. Scatchard plot showed that the addition of the binding mode of the complex to EB was uncompetitive inhibition compared with EB to DNA. So the binding mode of the complex with CT DNA was stable-electricity binding. Then the interaction of the complex with pBR322 was studied by the method of gel electrophoresis. The result showed that the complex could cleave pBR322 DNA effectively.
ISSN:1001-6538
1861-9541