Establishment and characterization of a cholangiocarcinoma cell line （RMCCA-1） from a Thai patient

• Published in: World journal of gastroenterology : WJG 2006, Vol.12 (40), p.6500-6506
• Main Author: Panthip Rattanasinganchan Kawin Leelawat Sa-ard Treepongkaruna Chintana Tocharoentanaphol Somboon Subwongcharoen Tuangporn Suthiphongchai Rutaiwan Tohtong
• Format: Article
• Language: English
• Subjects: 胆管癌
• ISSN: 1007-9327; 2219-2840

AIM： TO establish and characterize a new cell line derived from peripheral cholangiocarcinoma of a Thai patient. METHODS. The peripheral cholangiocarcinoma specimen surgically obtained from the patient was aseptically processed by washing and mincing before culturing in Ham＇s F12 medium containing 10% fetal bovine serum. After 3 mo, when the cell line has become homogeneous and stabilized, several features were investigated, including growth characteristics, immunofluorescence staining for cytokeratins, expression of tumor markers, chromosomal analysis by G-banding and multicolour fluorescence in situ hybridization （mFISH）, in vitro migration and invasion characteristics. RESULTS： The RMCCA-1 cell line has been established. These cells proliferated as a monolayer with a population doubling time of 48 h. Immunofluorescence staining showed positive staining for human cytokeratin 7 and 19 verifying the biliary epithelial origin. RMCCA-1 secreted carbohydrate antigen 19-9 （CA19-9）, but insignificant levels of carcinoembryonic antigen （CEA） and α-fetoprotein （AFP）. Chromosome analysis identified aneuploidy karyotypes with a modal chromosome number of 59. RMCCA-1 exhibited a low level of in vitro invasiveness, but a high degree of motility. The cell line exhibited a significant number of chromosomal aberrations as shown by mFISH and G-banding methods. CONCLUSION： A new cell line derived from peripheral cholangiocarcinoma of a Thai patient has been established. This cell line shows a low level of in vitro invasiveness, but a high degree of motility. It will serve as a valuable tool for further studies on tumor biology, molecular pathogenesis, metastatic mechanism and response to therapeutic drugs of cholangiocarcinoma.