Effect of ligand troglitazone on peroxisome proliferatoractivated receptor γ expression and cellular growth in human colon cancer cells

• Published in: World journal of gastroenterology : WJG 2006, Vol.12 (45), p.7263-7270
• Main Author: Mei Ming Jie-Ping Yu Xiang-Zhi Meng Yan-Hong Zhou Hong-Gang Yu He-Sheng kuo
• Format: Article
• Language: English
• Subjects: 细胞凋亡; 细胞周期; 结肠癌; 过氧物酶体
• ISSN: 1007-9327; 2219-2840

AIM： To investigate the effect of troglitazone on peroxisome proliferator-activated receptor γ （PPARγ） expression and cellular growth in human colon cancer HCT-116 and HCT-15 cells and to explore the related molecular mechanism. METHODS： Human colon cancer HCT-116 and HCT-15 cells cultured in vitro were treated with troglitazone. Reverse transcription-polymerase chain reaction （RT-PCR） and Western blot were employed to detect the effect of troglitazone on PPARy expression. The proliferative activity was determined by MTT assay, cell cycle and apoptosis were detected by flow cytometry. Apoptosisrelated genes, cell cycle regulatory genes and p53 were examined by RT-PCR and Western blot respectively. RESULTS： The expression of PPARy in colon cancer HCT-116 and HCT-15 cells was up-regulated by troglitazone. Troglitazone inhibited proliferation, induced apoptosis and cell cycle G1 arrest in colon cancer cells. Troglitazone induced p53 expression in HCT-116 cells, but not in HCT-15 cells. The down-regulation of survivin and bcl-2 was found in both cell lines and up-regulation of bax was found only in HCT-116 cells, being consistent with growth inhibition in HCT-116 cells but not in HCT-15 cells. Troglitazone increased expression of p21^WAF1/CIP1 （p21）, p27^KIP1 （p27） and reduced cyclin D1 in HCT-116 cells while only a minor decrease of cyclin D1 was found in HCT-15 cells. CONCLUSION： Troglitazone is an inductor of PPARγ in colon cancer cells and inhibits PPARγ-dependently proliferation, which may attribute to cell cycle G1 arrest and apoptosis in colon cancer cells. Troglitazone may induce p53-independent apoptosis and p53- dependent expression of p21 and p27. Depending on cell background, different activation pathways may exist in colon cancer cells.