Assignment of CCR 7 gene to chicken chromosome 27 by radiation hybrid panel mapping

• Published in: Journal of Zhejiang University. B. Science 2007, Vol.8 (5), p.314-317
• Main Author: TIAN Yong LU Li-zhi FU Yan TAO Zheng-rong SHEN Jun-da WANG De-qian YUAN Ai-ping YIN Zhao-zheng
• Format: Article
• Language: English
• Subjects: 27号染色体; CCR7基因; 放射杂交板; 遗传作图
• ISSN: 1673-1581; 1862-1783

The protein encoded by CC chemokine receptor 7 （CCR7） is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus （EBV）, and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. To map the CCR7 gene in chicken chromosome, a 6000 rads chicken-hamster radiation hybrid panel （ChickRH6） was used. PCR of samples from ChickRH6 revealed that the location of CCR7 gene is linked to the maker SEQ0347 （6 cR away） with LOD score of 16.6 and that the marker SEQ0347 is located on chromosome 27 at 27 cR of RH （radiation hydrid） map. We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken CCR7 gene, and found that the assembled contig shared a high percentage of similarity with that of the human gene.