First report of an endophyte （Diaporthe phaseolorum var. sojae） from Kandelia candel

• Published in: Journal of forestry research 2008, Vol.19 (4), p.277-282
• Main Author: CHENG Zhong-shan TANG Wen-cheng XU Shu-lan SUN Shi-feng Huang Bo-You Yan Xi CHEN Qi-jin LINYong-cheng
• Format: Article
• Language: English
• Subjects: 内生植物; 形态学; 系统发生学; 红树林
• ISSN: 1007-662X; 1993-0607

Mangrove endophytic fungus 1893 was isolated from Kandelia candel from an estuarine mangrove on the South China Sea Coast Two new lactones 1893A and 1893B, together with other known compounds, have been isolated from its fermentation broth. To classify the endophyte correctly for further industrial application, a combination of morphological and molecular techniques was used to approach its identity. The endophyte was compared with similar species having trichogynes or trichogyne-like hyphae which apparently fused with an- theridium-like hyphae, and perithecia initials developing from an ascogonial coil surrounded by enveloping hyphae in early developmental stages on pure culture. Further morphological characteristics on host and non-host were used for comparison with similar species when the endophyte was cultivated on leaves ofKandelia candel and Mangifera indica, respectively, which resulted in classifying the endophyte as a Phomopsis specics. The ITS sequence of rDNA was used to infer its phylogenetic relationships with Phomopsis species that resembled the strain in morphology or ecology. Finally, the endophyte was identified as Diaporthe phaseolorum var. sojae based on morphological and molecular evidence. Our study is a first report ofDiaporthephaseolorum var. sojae isolated from mangrove Kandelia candel.