Association of Fas/Apol gene promoter （-670 A/G） polymorphism in Tunisian patients with IBD

• Published in: World journal of gastroenterology : WJG 2009 (29), p.3643-3648
• Main Author: Walid Ben Aleya Imen Sfar Leila Mouelhi Houda Aouadi Mouna Makhlouf Salwa Ayed-Jendoubi Samira Matri Azza Filali Taoufik Najjar Taeib Ben Abdallah Khaled Ayed Yousr Gorgi
• Format: Article
• Language: English
• Subjects: Fas; 基因启动子; 多态性分析; 溃疡性结肠炎; 突尼斯; 限制性片段长度多态性
• ISSN: 1007-9327; 2219-2840

AIM： To detect a possible association between the polymorphism of the （-670 A/G） Fas/Apol gene promoter and susceptibility to Crohn＇s disease （CD） and ulcerative colitis （UC） in the Tunisian population. METHODS： The （-670 A/G） Fas polymorphism was analyzed in 105 patients with CD, 59 patients with UC, and 100 controls using the polymerase chain reaction restriction fragment length polymorphism method. RESULTS： Significantly lower frequencies of the Fas -670 A allele and A/A homozygous individuals were observed in CD and UC patients when compared with controls. Analysis of （-670 A/G） Fas polymorphism with respect to sex in CD and UC showed a significant difference in A/A genotypes between female patients and controls （P corrected = 0.004 ＂in CD patients＂ and P corrected = 0.02 ＂in UC patients＂, respectively）. Analysis also showed a statistically significant association between genotype AA of the （-670 A/G） polymorphism and the ileum localization of the lesions （P corrected = 0.048） and between genotype GG and the colon localization （P corrected = 0.009）. The analysis of IBD patients according to clinical behavior revealed no difference. CONCLUSION： Fas-670 polymorphism was associated with the development of CD and UC in the Tunisian population.