Evaluation of Biological Contributing to Biofilm Characteristics of Bacteria Formation

• Published in: Pedosphere 2009 (5), p.554-561
• Main Author: LI Meng-Ying ZHANG Ji LU Peng XU Jing-Liang LI Shun-Peng
• Format: Article
• Language: English
• Subjects: 废水处理系统; 形成能力; 恶臭假单胞菌; 生物膜特性; 生物评价; 细菌总数; 蜡样芽胞杆菌; 豚鼠气单胞菌
• ISSN: 1002-0160; 2210-5107

Biofilm-associated microorganisms play crucial roles in terrestrial and aquatic nutrient cycling and in the biodegradation of environmental pollutants. Biofilm formation was determined for a total of 18 bacterial isolates obtained from the biofilms of wastewater treatment systems and of little carpolite in soil. Among these isolates, seven showed strong biofilm-forming capacity. The phylogenetic affiliation of the isolates showing high biofilm formation capacity was determined through 16S rDNA sequencing and the isolates were grouped into 7 bacterial species including Pseudornonas sp., Pseudomonas putida, Aeromonas caviae, Bacillus cereus, Pseudornonas plecoglossicida, Aeromonas hydrophila, and Comamonas testosteroni. The biofilm-forming capacity was closely related with flagella, exopolysaccharide, and extracellular protein. According to the coefficient of determination, the relative importance of the five biological characteristics to biofilm formation was, in order from greatest to least, exopolysaccharide 〉 flagella 〉 N-acyl-homoserine lactones （AHLs） signaling molecules 〉 extracellular protein 〉 swarming motility.