Identification of MicroRNAs in Wild Soybean （ Glycine soja）

MicroRNAs （miRNAs） play important roles in post-transcriptional gene silencing by directing target mRNA cleavage or translational inhibition. Currently, hundreds of miRNAs have been identified in plants, but no report has been published of wild soybean （Glycine soja Sieb）. We constructed a small-RNA...

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Bibliographic Details
Published in:Journal of integrative plant biology 2009 (12), p.1071-1079
Main Author: Rui Chen Zheng Hu Hui Zhang
Format: Article
Language:English
Subjects:
cDNA末端快速扩增
microRNA
miRNAs
RNA二级结构
小分子RNA
标签数据库
野生大豆
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Summary:MicroRNAs （miRNAs） play important roles in post-transcriptional gene silencing by directing target mRNA cleavage or translational inhibition. Currently, hundreds of miRNAs have been identified in plants, but no report has been published of wild soybean （Glycine soja Sieb）. We constructed a small-RNA library consisting of 2 880 sequences with high quality, in which 1 347 were 19-24 nt in length. By utilizing the miRNA, Rfam and domesticated soybean expressed sequence tag database, we have analyzed and predicted the secondary structure of these small RNAs. As a result, t5 conserved miRNA candidates belonging to eight different families and nine novel miRNA candidates comprising eight families were identified in wild soybean seedlings. All these miRNA candidates were validated by northern blot and the novel candidates expressed in a tissue-specific manner. Furthermore, putative target genes were predicted for novel miRNA candidates and two of them were verified by 5＇-rapid amplification of cDNA ends experiments. These results provided useful information for miRNA research in wild soybean and plants.
ISSN:1672-9072
1744-7909