Farnesylcysteine Lyase Regulation of Abscisic Arabidopsis is Involved in Negative Acid Signaling in

The Arabidopsis FCLY gene encodes a specific farnesylcysteine （FC） lyase, which is responsible for the oxidative metabolism of FC to farnesal and cysteine. In addition, fcly mutants with quantitative decreases in FC lyase activity exhibit an enhanced response to ABA. However, the enzymological prope...

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Bibliographic Details
Published in:Molecular plant 2010 (1), p.143-155
Main Author: David H. Huizinga Ryan Denton Kelly G. Koehler Ashley Tomasello Lyndsay Wood Stephanie E. Sen Dring N. Crowell
Format: Article
Language:Chinese
Subjects:
信号
反应活性
基因编码
拟南芥
脱落酸
裂解酶
负调控
酸性
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Summary:The Arabidopsis FCLY gene encodes a specific farnesylcysteine （FC） lyase, which is responsible for the oxidative metabolism of FC to farnesal and cysteine. In addition, fcly mutants with quantitative decreases in FC lyase activity exhibit an enhanced response to ABA. However, the enzymological properties of the FCLY-encoded enzyme and its precise role in ABA signaling remain unclear. Here, we show that recombinant Arabidopsis FC lyase expressed in insect cells exhib- its high selectivity for FC as a substrate and requires FAD and molecular oxygen for activity. Arabidopsis FC lyase is also shown to undergo post-translational N-glycosylation. FC, which is a competitive inhibitor of isoprenylcysteine methyltransferase （ICMT）, accumulates in fcly mutants. Moreover, the enhanced response of fcly mutants to ABA is reversed by ICMToverexpression. These observations support the hypothesis that the ABA hypersensitive phenotype of fcly plants is the result of FC accumulation and inhibition of ICMT.
ISSN:1674-2052
1752-9867