Establishment of an ELISA to Detect Kaposi＇s Sarcoma-associated Herpesvirus Using Recombinant ORF73

Kaposi＇s sarcoma-associated herpesvirus （KSHV） is causally related to Kaposi＇s sarcoma （KS）, primary effusion lymphoma （PEL） and a proportion of cases of multicentric Castleman＇s disease （MCD）. The ORF73 protein was cloned into pQE80L-orf73 and expressed in E.coli and purified. The expressed recombi...

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Bibliographic Details
Published in:Virologica Sinica 2010 (3), p.168-176
Main Author: Xin-xing OUYANG Bi-shi FU Bao-lin LI Yan ZENG Fan-hong XU Lin-ding WANG
Format: Article
Language:English
Subjects:
ELISA检测
ELISA法
PAGE电泳
因果关系
基因蛋白
疱疹病毒
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Summary:Kaposi＇s sarcoma-associated herpesvirus （KSHV） is causally related to Kaposi＇s sarcoma （KS）, primary effusion lymphoma （PEL） and a proportion of cases of multicentric Castleman＇s disease （MCD）. The ORF73 protein was cloned into pQE80L-orf73 and expressed in E.coli and purified. The expressed recombinant ORF73 was identified by sodium dodecyl sulfatepolyacrylamide gel electrophoresis （SDS-PAGE）. A protein of about 27 kDa was expressed as expected. Western Blotting showed that the purified recombinant ORF73 reacted with KSHV positive serum. The immunogenicity of the recombinant ORF73 was further analysed by ELISA and the optimal conditions were determined. The ORF73 ELISA was used to compare the KSHV seroprevalence between Hubei and Xinjiang Han people. The Han people in Xinjiang have significantly higher KSHV seroprevalence than their counterparts in Hubei （6.7% vs 2.9%, P = 0.005）.
ISSN:1674-0769
1995-820X