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Biodegradation of Pentachloronitrobenzene by I.abrys portucalensis pcnb-21 Isolated from Polluted Soil

A bacterial strain, pcnb-21, capable of degrading pentaehloronitrobenzene (PCNB) under aerobic and anoxic conditions, was isolated from a long-term PCNB-polluted soil by an enrichment culture technique and identified as Labrys portucalensis based upon its morphological, physiological and biochemical...

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Published in:Pedosphere 2011, Vol.21 (1), p.31-36
Main Author: LI Rong ZHENG Jin-Wei NI Bin CHEN Kai YANG Xiu-Juan LI Shun-Peng JIANG Jian-Dong
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description A bacterial strain, pcnb-21, capable of degrading pentaehloronitrobenzene (PCNB) under aerobic and anoxic conditions, was isolated from a long-term PCNB-polluted soil by an enrichment culture technique and identified as Labrys portucalensis based upon its morphological, physiological and biochemical properties, as well as 16S rRNA gene sequence analysis. Effects of different factors, such as temperature and pH, on PCNB biodegradation were studied. Strain pcnb-21 efficiently degraded PCNB at temperatures from 20 to 30 ℃ and initial pH values from 4 to 7, which might be the first time that a Labrys strain was found capable of eflClciently degrading PC1NB. The degradation of PCNB was affected by oxygen, and the degradation decreased with increasing aeration. Exogenous electron donors such as glucose, lactic acid and succinic acid promoted the biodegradation of PCNB, while electron acceptors such as sodium nitrite, sodium sulfate, sodium nitrate and sodium sulfate inhibited PCNB biodegradation. The degradation of PCNB in sterile and non-sterile soils by a green fluorescent protein (GFP)-labeled strain, pcnb-21-gfp, was also studied. Cells of pcnb-21-gfp efficiently degraded 100 mg kg-1 PCNB in sterile and non-sterile soils and could not be detected after 42 days. Strain pcnb-21 might be useful in bioremediating PCNB-polluted soils and environment.
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subjects 五氯硝基苯
初始pH值
基因序列分析
污染土壤
生物降解
生理生化基础
绿色荧光蛋白
title Biodegradation of Pentachloronitrobenzene by I.abrys portucalensis pcnb-21 Isolated from Polluted Soil
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