Suppressing progress of pancreatitis through selective inhibition of NF-κB activation by using NAC

Objective:To explore the characteristics of NF-κB activation in the progress of pancreatitis，the relationship with expression of TNF-α in the inflammatory reaction, and prevent the exacerbation of pancreatitis by using NAC.Method:Forty-eight rats were divided into three groups: therapy (group C), pa...

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Bibliographic Details
Published in:Journal of Zhejiang University. Science 2004, Vol.5 (4), p.477-482
Main Author: 赵志成 郑树森 陈文亮 王选 齐莹
Format: Article
Language:English
Subjects:
NF-κB
活化作用
炎性反应
胰腺炎
选择性抑制
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Summary:Objective:To explore the characteristics of NF-κB activation in the progress of pancreatitis，the relationship with expression of TNF-α in the inflammatory reaction, and prevent the exacerbation of pancreatitis by using NAC.Method:Forty-eight rats were divided into three groups: therapy (group C), pancreatitis (group B) and control (group A).NAC served as the inhibitor of NF-κB activation.In the time intervals of 1.5, 3.0, 6.0, 12.0 hour, NF-κB activation was detected with flow cytometry (FCM) and the expression of TNF-α mRNA and protein with in situ hybridization (ISH) and enzyme-linked immuno-sorbent assay (ELISA) respectively. Meanwhile, the level of lipase and amylase in the serum was assayed and the pathological change was evaluated. Result:NF-κB activation in the pancreatitis group was higher than that in the control group (P
ISSN:1009-3095