Expression change of TNF-αin myocardium and hepatic tissue of rats with compound stress of hyperthermia and Hpopolysaccharide

• Published in: 亚太热带医药杂志：英文版 2013 (4), p.300-304
• Main Author: Xiao-Jun Zhang Ji Yue Ting-Bao Zhao
• Format: Article
• Language: English
• Subjects: Hyperthermia; Immunohistochemistry; inflammatory; Lipopolysaccharide; response; Syndrome; Systemic; TNF-α
• ISSN: 1995-7645; 2352-4146

<正>Objective:To investigate the expression characteristics of TNF-αin myocardium and hepatic tissue of rats with compound stress of hyperthermia and lipopoiysaccharide(LPS).Methods: Male SPF Wistar rats were randomly divided into room temperature+physiological saline group (Croup C),hyperthermia+physiological saline group(Croup II),room temperature+LPS group(Croup L) and hyperthermia+LPS group(Croup HL).The rats were put in simulated climate cabin.Croup HL and Croup H were exposed in the environment at a dry bulb temperature(TDB) of(35.0±0.5)℃,while Croup L and Croup C were exposed in the environment at a TDB of(26.0±0.5)℃. The rats in Croup HL and Croup L were given tail intravenous injection of LPS 10 mg/kg,while the rats in Croup H and Croup C were given tail intravenous injection of 9 g/L NaCl 10 ml/kg. After the stress,immunohistochemical SABC staining method was used to detect the expression characteristics of TNF-αin myocardium and hepatic tissue of rats,and those rats were given routine pathological examinations.Results:The expression of TNF-αin myocardium and hepatic tissue in Croup HL was enhanced remarkably,and the tissue damages of Croup HL were severest.Conclusions:The eardiotoxieity and hepatotoxicity caused by compound stress of hyperthermia and LPS is closely related to the expression of TNF-α.