Msx2 plays a critical role in lens epithelium cell cycle control

· AIM: To investigate the effects of on lens epithelium cell cycle, and evaluate the changes of the proliferation, apoptosis of lens epithelium cells. ·METHODS: Mice lens epithelium cells were cultured and transfected with and control. deficient mice ( ) lens tissue were isolated. Lens tissue and tr...

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Published in:国际眼科杂志：英文版 2013 (3), p.276-279
Main Author: Jiang-Yue Zhao Feng-Feng Zhuang Hong-Yan Wang Di Wu Jin-Song Zhang
Format: Article
Language:English
Subjects:
cellcycle
lensepitheliumcell
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Summary:· AIM: To investigate the effects of on lens epithelium cell cycle, and evaluate the changes of the proliferation, apoptosis of lens epithelium cells. ·METHODS: Mice lens epithelium cells were cultured and transfected with and control. deficient mice ( ) lens tissue were isolated. Lens tissue and transfected cells were prepared for mRNA extraction using Trizol reagent. and expression were evaluated by real -time RT -PCR. BrdU incorporation and apoptosis rate were investigated by immunofluorescence and flow cytometry analysis. ·RESULTS: After transfected with , lens epithelium cells failed to incorporate BrdU and anti phospho -histone -3 immunofluorescence failed to detect cell nuclei which GFP were positive. over expression resulted in increasing apoptosis rate in lens epithelium cells. and expression increased significantly in knockout mice by real time RT -PCR quantization and expression decreased significantly in overexpressed cell. · CONCLUSION: has the effect of inhibiting proliferation and differentiation, triggering apoptosis on mice lens epithelium cells. ·
ISSN:2222-3959
2227-4898