Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells

·AIM: To analyze proteomic and signal transduction alterations in irradiated melanoma cells. ·METHODS: We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification....

Full description

Saved in:
Bibliographic Details
Published in:国际眼科杂志：英文版 2013 (3), p.286-294
Main Author: Lu-Bin Yan Kai Shi Zhi-Tong Bing Yi-Lan Sun Yang Shen
Format: Article
Language:English
Subjects:
2D-LC-MS/MS
acids
amino
analysis
cell
interaction
irradiation
isotope
labeling
melanoma
protein-protein
proteomic
stable
with
X-ray
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:·AIM: To analyze proteomic and signal transduction alterations in irradiated melanoma cells. ·METHODS: We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein protein interaction was used to analyze relationships among proteins. · RESULTS: Energy metabolism protein levels were significantly different in glycolysis and not significantly different in oxidative phosphorylation after irradiation. Conversely, tumor suppressor proteins related to cell growth and development were downregulated, and those related to cell death and cell cycle were upregulated in irradiated cells. · CONCLUSION: Our results indicate that irradiation induces differential expression of the 29 identified proteins closely related to cell survival, cell cycle arrest, and growth inhibition. The data may provide new insights into the pathogenesis of uveal melanoma and guide appropriate radiotherapy. ·
ISSN:2222-3959
2227-4898