Elevated Circulating Levels of Osteopontin Are Associated with Metastasisin Advanced Non-Small Cell Lung Cancer

• Published in: 中国癌症研究：英文版 2011, Vol.23 (1), p.64-68
• Main Author: Yong Liang Hui Li Bin Hu Xing Chen Jin-bai Miao Tong Li Bin You Qi-rui Chen Yi-li Fu Yang Wang Sheng-cai Hou
• Format: Article
• Language: English
• Subjects: mRNA表达水平; 循环水; 抗肿瘤治疗; 晚期; 蛋白水平; 非小细胞肺癌; 骨桥蛋白; 高架
• ISSN: 1000-9604; 1993-0631

Objective：To investigate the relationship between postoperative metastasis and circulating levels of osteopontin in non-small cell lung cancer（NSCLC）.Methods：The expression of osteopontin mRNA were detected with RT-PCR technique.The circulating levels of osteopontin were measured through ELASA in 46 NSCLC cases that had not been received any anti-cancer treatment at the time of sampling.The tissues from fifteen patients with benign pulmonary diseases were studied as control group.Results：The overall median mRNA expression level of osteopontin was approximately 70-fold higher in tumor tissues than in matched normal lung tissues（P0.001）.Over-expression of osteopontin mRNA was significantly associated with clinical stage（P=0.009）.Advanced disease states had higher circulating level of osteopontin（stage I＋II versus stage III＋VI）.In multivariate analysis,stage was the only independent factor influencing circulating levels of osteopontin.All patients were followed up for 12 months,2 of the 46 patients with both osteopontin mRNA expression and elevated plasma osteopontin levels had local recurrence and 10 had distant metastasis.There was a significant difference in the osteopontin levels between metastasis group and non-metastasis group.Conclusion：Preoperative plasma levels of osteopontin are significantly associated with post-operative metastasis in advanced NSCLC.