Bovine Herpesvirus I Protein blCPO Represses the Transcription of blSGIS in Fetal Bovine Lung Cells

The ubiquitin-like modifier bISG15 is an antiviral protein found in fetal bovine lung （FBL） cells. Bovine Herpesvirus 1（BHV-1）, which is a viral pathogen of cattle, can infect FBL cells and induce cytopathic effects. Real-time PCR assays showed that BHV-l＇s infection could repress the basal or induc...

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Published in:中国病毒学：英文版 2011, Vol.26 (6), p.403-408
Main Author: Chang Liu Xiao-hong Kong Wen-tao Qiao Yun-qi Geng
Format: Article
Language:English
Subjects:
PCR检测
抗病毒蛋白
牛疱疹病毒
病毒感染
细胞病变效应
肺细胞
胎牛
转录
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Summary:The ubiquitin-like modifier bISG15 is an antiviral protein found in fetal bovine lung （FBL） cells. Bovine Herpesvirus 1（BHV-1）, which is a viral pathogen of cattle, can infect FBL cells and induce cytopathic effects. Real-time PCR assays showed that BHV-l＇s infection could repress the basal or inducible transcription of bISG15 in FBL cells. It demonstrates that this repression effect depends on BHV-1 viral infection and new protein synthesis. Our previous work showed that bIRF-3 was the key factor in the stimulation ofbISG15 in FBL cells, so the effect of BHV-1 viral protein on bIRF-3 activating the promoter of bISG15 was confirmed. The luciferase assay showed the BHV-1 viral protein bICP0 inhibited the activation of bISG15 promoter stimulated by bIRF-3. Taken together, our work suggested that BHV-1 had some molecular mechanism to resist the cellular bISG15＇s antiviral functions.
ISSN:1674-0769
1995-820X