Phylogenetic diversity of TypeⅠpolyketide synthase genes from sediments of Ardley Island in Antarctica

The diversity of modular polyketide synthase （PKS） genes in sediments of Ardley Island in Antarctica, was studied by restriction fragment length polymorphism （RFLP） analysis. Phylogenetic analysis of 14 amino acid （AA） sequences indicates that the identified ketosynthase （KS） domains were clustered...

Full description

Saved in:
Bibliographic Details
Published in:海洋学报：英文版 2011, Vol.30 (6), p.104-111
Main Author: ZHAO Jing YANG Ning CHEN Xinglin JIANG Qingru ZENG Runying
Format: Article
Language:English
Subjects:
PKS基因
南极
沉积物类型
系统发育多样性
聚酮合酶
酶基因
阿德雷岛
限制性片段长度多态性
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The diversity of modular polyketide synthase （PKS） genes in sediments of Ardley Island in Antarctica, was studied by restriction fragment length polymorphism （RFLP） analysis. Phylogenetic analysis of 14 amino acid （AA） sequences indicates that the identified ketosynthase （KS） domains were clustered with those from diverse bacterial groups, including Cyanobacteria, γ-Proteobacteria, Actinobacteria, Firmicutes, and some unidentified microorganisms from marine sponge, bryozoan and other environmental samples. The obtained KS domains showed 43%–81% similarity at the AA level to reference sequences in GenBank. Six identified KS domains showed diverse sequences of the motif （VQTACSTS） that was used to identify the hybrid PKS/nonribosomal peptide synthetase （NRPS） enzyme complex, and formed a new branch. These results reveal a high diversity and novelty of PKS genes in antarctic sediments.
ISSN:0253-505X
1869-1099