Electro-oxidation of Glycerol from Cooking Palm Oil by Dehydrogenase Enzymes Immobilized on Poly（o-phenylenediamine） Modified Electrodes

This study reported on the electrochemical oxidations of glycerol, obtained from cooking palm oil hydrolyzed using commercial lipase, by two monoenzyme systems： （1） glycerol dehydrogenase （GDH）; and （2） alcohol dehydrogenase （ADH） immobilized within Nation layer on the surface of poly（o-phenylenedia...

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Bibliographic Details
Published in:材料科学与工程：中英文B版 2011, Vol.1 (5), p.603-610
Main Author: Zahraa A. Jarjes Mohammed Razip Samian Sulaiman A.B. Ghani
Format: Article
Language:Chinese
Subjects:
乙醇脱氢酶
修饰电极
可逆电极过程
固定化
棕榈油
甘油脱氢酶
电氧化
邻苯二胺
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Summary:This study reported on the electrochemical oxidations of glycerol, obtained from cooking palm oil hydrolyzed using commercial lipase, by two monoenzyme systems： （1） glycerol dehydrogenase （GDH）; and （2） alcohol dehydrogenase （ADH） immobilized within Nation layer on the surface of poly（o-phenylenediamine） （PoPD） modified carbon cloth electrode. Another system studied was a bienzyme system of ADH and aldehyde dehydrogenase （AIdDH） immobilized similarly on the substrate electrode described earlier. The later was aimed for a possible study of multi-step oxidation of glycerol. Voltammetric studies over potential ranges of-1.5 to 0.1V vs. Ag/AgCl （3 M KCl）, have showed that these electrodes displayed mostly quasi-reversible type of electrode processes for the oxidation of glycerol. Various parameters have also been examined for experimental optimum conditions. In general, the maximum oxidative current density for all electrodes was obtained at pH 7 with weight of lipase 0.1-0.15 g and concentration of nicotine aden
ISSN:2161-6221