Compound Danshen tablets downregulate amyloid protein precursor mRNA expression in a transgenic cell model of Alzheimer＇s disease Effects and a comparison with donepezil

After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyl...

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Bibliographic Details
Published in:中国神经再生研究：英文版 2012, Vol.7 (9), p.659-663
Main Author: Ren＇an Qin Desheng Zhou Jiajun Wang Hua Hu Yang Yang Xiaoxuan Yao Xiaopeng Sun
Format: Article
Language:English
Subjects:
HEK293细胞
前体mRNA
复方丹参片
淀粉样蛋白
细胞模型
转基因
阿尔茨海默氏病
阿尔茨海默氏症
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Summary:After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer＇s disease, with similar effects to donepezil.
ISSN:1673-5374