Intracerebroventricular transplanted bone marrow stem cells survive and migrate into the brain of rats with Parkinson＇s disease

• Published in: 中国神经再生研究：英文版 2012, Vol.7 (13), p.978-984
• Main Author: Ping Gu Zhongxia Zhang Dongsheng Cui Yanyong Wang Lin Ma Yuan Geng Mingwei Wang
• Format: Article
• Language: English
• Subjects: Morris水迷宫; 免疫组织化学; 大鼠脑; 帕金森氏症; 干细胞移植; 细胞迁移; 骨髓基质干细胞; 骨髓干细胞
• ISSN: 1673-5374

In this study, 6-hydroxydopamine was stereotaxically injected into the right substantia nigra compact and ventral tegmental area of rats to establish Parkinson＇s disease models. The rats then received a transplantation of bone marrow stromal cells that were previously isolated, cultured and labeled with 5-bromo-2＇-deoxyuridine in vitro. Transplantation of the bone marrow stromal cells significantly decreased apomorphine-induced rotation time and the escape latency in the Morris water maze test as compared with rats with untreated Parkinson＇s disease. Immunohistochemical staining showed that, 5-bromo-2＇-deoxyuridine-immunoreactive cells were present in the lateral ventricular wall and the choroid plexus 1 day after transplantation. These immunoreactive cells migrated to the surrounding areas of the lateral cerebral ventricle along the corpus callosum. The results indicated that bone marrow stromal cells could migrate to tissues surround the cerebral ventricle via the cerebrospinal fluid circulation and fuse with cells in the brain, thus altering the phenotype of cells or forming neuron-like cells or astrocytes capable of expressing neuron-specific proteins. Taken together, the present findings indicate that bone marrow stromal cells transplanted intracerebroventricularly could survive, migrate and significantly improve the rotational behavior and cognitive function of rats with experimentally induced Parkinson＇s disease.