Cell Viability of Byrsonima intermedia A Juss Calli

• Published in: 农业科学与技术：B 2012, Vol.2 (6), p.713-720
• Main Author: Luciano Coutinho Silva Renato Paiva Daiane Peixoto Vargas Diogo Pedrosa Correa da Silva Rairys Cravo Herrera Sandro Barbosa Antonio Paulino da Costa Netto
• Format: Article
• Language: Chinese
• Subjects: MS培养基; 亚文化; 分光光度法分析; 愈伤组织; 细胞活力; 细胞活性; 继代培养; 药理特性
• ISSN: 2161-6264

Byrsonima intermedia A Juss. is a species with pharmacological properties from the Brazilian Cerrado that shows difficulties related to sexual propagation. Research on cell viability may provide useful information for the selection of cells with embryogenic potential during the callus culture, Within this context, our research is aimed at establishing the cell viability of calli from Byrsonima intermedia leaf segments. The calli went through three subculture phases, of 60 days each, in MS medium with 0.09 M sucrose, 0.6% agar, pH 5.8 and 4.52 laM 2,4-D. The calli were stored in dark conditions and samples were collected every 10 days from each subculture for viability tests with fluorescein 3,6-diacetate （FDA） and 2,3,5-triphenyltetrazolium chloride （TTC）. The staining methods allowed quantifying cell viability in each subculture. The best results from the FDA tests were obtained at 21, 25 and 29 days for the first, second and third subcultures respectively, with 53,86%, 61.88% and 53.73% viable cells. Regard