Fluphenazine hydrochloride radical and precise method to determine capacity of fruit cation assay： in vitro total extracts A new, rapid antioxidant

• Published in: 中国化学快报：英文版 2012, Vol.23 (11), p.1271-1274
• Main Author: Muhammad Nadeem Asghar Qadeer Alam Sharoon Augusten
• Format: Article
• Language: English
• Subjects: 体外; 奋乃静; 容量; 总抗氧化能力; 抽取; 水果; 盐酸盐; 离子分析
• ISSN: 1001-8417; 1878-5964

A new procedure based on generation and subsequent reduction of orange-colored fluphenazine hydrochloride radical （FPH^＋） is presented for the screening of total antioxidant capacity （TAC） of various fruit matrices. The FPH^＋ was obtained by mixing fluphenazine hydrochloride with persulfate （final concentration 2 mmol/L and 0.05 mmol/L, respectively） in 3 mol/L H2SO4 with constant shaking for 5 min. The solution formed showed maximum absorption as 0.8 ＋ 0.02 at 500 nm in first-order derivative spectrum. The percent inhibition of the solution increased linearly on addition of increasing amounts of standard antioxidants i.e., ascorbic acid etc. The TACs of sample citrus juices were calculated in terms of ascorbic acid equivalents （AAEs） by comparing their inhibition curves with that of ascorbic acid. Comparison of AAE values of different commercial orange juices using the newly developed FPH^＋ assay and the well-known ABTS/K2S2Os and DMPD/FeC13 assays indicated the precision and comparable sensitivity of the method. The proposed procedure is quick, economical, and more precise and gives results comparable to contemporary assays.