Abundance and diversity of candidate division JSl- and Chloroflexi-related bacteria in cold seep sediments of the northern South China Sea

• Published in: 地球科学前沿：英文版 2012, Vol.6 (4), p.373-382
• Main Author: Yong ZHANG Xin SU Fang CHEN Lu JIAO Hongchen JIANG Hailiang DONG Guochun DING
• Format: Article
• Language: English
• Subjects: 中国; 冷泉; 分割; 南海北部; 多样性; 深海沉积物; 相对丰度; 细菌总数
• ISSN: 2095-0195; 2095-0209

Candidate division JS 1-and Chloroflexi-related bacteria are ubiquitous in various deep marine sediments worldwide, yet almost nothing is known about their abundance and diversity in cold seep sediments. Here, we investigated the abundance and diversity of JS1- and Chloroflexi-related bacteria in a cold seep marine sediment core collected from the northern South China Sea （SCS） with the employment of quantitative polymerase chain reaction （qPCR） and 16S rRNA gene phylogenetic analyses. The qPCR results showed that 16S rRNA gene copies per gram of sediments for the total bacteria and JS 1- and Chloroflexi-related bacteria were at magnitudes of 108 and 106, respectively. The relative abundance of JS 1- and Chloroflexi-related 16S rRNA genes to that of total bacteria was 0.07%-8.78% throughout the core. Phyloge- netic analyses showed that the JS-1 related clone sequences were dominant throughout the core. Our study provided insights into abundance and diversity of JS 1- and Chloroflexi-related bacteria in the northern SCS cold seep sediments.