Effects of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis

• Published in: 浙江大学学报：B卷英文版 2013, Vol.14 (3), p.194-194
• Main Author: Jing-min Ou Xi-ping Zhang Cheng-jun Wu Di-jiong Wu Ping Yan
• Format: Article
• Language: English
• Subjects: ICAM-1; Toll样受体4; 丹参; 器官组织; 地塞米松; 大鼠; 细胞间黏附分子-1; 重症急性胰腺炎
• ISSN: 1673-1581; 1862-1783

Objective： To investigate the protective effects and mechanisms of action of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis （SAP）. Methods： The rats were divided into sham-operated, model control, dexamethasone treated, and Salvia miltiorrhiza treated groups. At 3, 6, and 12 h after operation, the mortality rate of different groups, pathological changes, Bcl-2-associated X protein （Bax） and nuclear factor-KB （NF-kB） protein expression levels in multiple organs （the pancreas, liver, kidneys, and lungs）, toll-like receptor 4 （TLR-4） protein levels （only in the liver）, intercellular adhesion molecule 1 （ICAM-1） protein levels （only in the lung）, and terminal deoxynucleotidy transferase mediated deoxyuridine triphosphate （dUTP） nick end labeling （TUNEL） staining expression levels, as well as the serum contents of amylase, glutamate-pyruvate transaminase （GPT）, glutamic-oxaloaeetic transaminase （GOT）, blood urea nitrogen （BUN）, and creatinine （CREA） were observed. Results： The mortality rate of the dexamethasone treated group was significantly lower than that of the model control group （P〈0.05）. The patho-logical changes in multiple organs in the two treated groups were relieved to different degrees （P〈0.05 and P〈0.01, respectively）, the expression levels of Bax and NF-KB proteins, and apoptotic indexes of multiple organs were reduced （P〈0.05 and P〈0.01, respectively）. The contents of amylase, GPT, GOT, BUN, and CREA in the two treated groups were significantly lower than those in model control groups （P〈0.05 and P〈0.01, respectively）. The expression level of ICAM-1 protein in the lungs （at 3 and 12 h） in the dexa-ethasone treated group was significantly lower than that in the Salvia miltiorrhiza treated group （P〈0.05）. The serum contents of CREA （at 12 h） and BUN （at 6 h） of the Salvia miltiorrhiza treated group were sig- nificantly lower than those in the dexamethasone treated group （P〈0.05）. Conclusions： Both dexamethasone and Salvia miltiorrhiza can reduce the inflammatory reaction, regulate apoptosis, and thus protect multiple organs of rats with SAP.