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Salvianolate inhibits reactive oxygen species production in H2O2-treated mouse cardiomyocytes in vitro via the TGFI] pathway

Aim: To investigate the effects of salvianolate, a water-soluble active compound from Salvia miltiorrhiza Bunge, on reactive oxygen species (ROS) production in mouse cardiomyocytes in vitro. Methods: Primary ventricular cardiomyocytes were prepared from neonatal mouse. The cell viability was determi...

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Published in:中国药理学报:英文版 2013 (4), p.496-500
Main Author: Ai-hua FEI Qing CAO Shu-yan CHEN Hai-rong WANG Fei-long WANG Shu-ming PAN Zhao-fen LIN
Format: Article
Language:English
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Summary:Aim: To investigate the effects of salvianolate, a water-soluble active compound from Salvia miltiorrhiza Bunge, on reactive oxygen species (ROS) production in mouse cardiomyocytes in vitro. Methods: Primary ventricular cardiomyocytes were prepared from neonatal mouse. The cell viability was determined using MTT assay. Culture medium for each treatment was collected for measuringthe levels of NO, iNOS, total antioxidant capacity (TAOC) and transforming growth factor 131 (TGFI31). TGFI31 and Smad2/3 expression in the cells was detected with Western blotting. Results: H202 (1.25 mmoVL) did not significantly affect the cell viability, whereas the high concentration of salvianolate (5 E/L) alone dramatically suppressed the cell viability. Treatment of the cells with H202 (1.25 mmol/L) markedly increased ROS and iNOS production, and decreased the levels of NO, TAOC and TGFI31 in the culture medium. Furthermore, the H202 treatment significantly increased TGFI31 and Smad2/3 expression in the cells. Addition of salvianolate (O.05, O.1, and 0.5 E/L) concentration-dependently reversed the H202-induced alterations in the culture medium; addition of salvianolate (0.05 E/L) reversed the H202-induced increases of TGFI31 and Smad2/3 expression in the cells. Blockage of TGFI31 with its antibody (1 mE/L) abolished the above mentioned effects of salvianolate. Conclusion: Salvianolate inhibits ROS and iNOS production and increases TAOC and NO levels in H202-treated cardiomyocytes in vitro via downregulation of Smad2/3 and TGFβ1 expression. High concentration of salvianolate causes cytotoxicity in mouse cardiomyocytes.
ISSN:1671-4083
1745-7254