Cloning and Functional Analysis of Lycopene ε-Cyclase （lbL C Ye） Gene from Sweetpotato, Ipomoea batatas （L.） Lam

• Published in: 农业科学学报：英文版 2013 (5), p.773-780
• Main Author: YU Ling ZHAI Hong CHEN Wei HE Shao-zhen LIU Qing-chang
• Format: Article
• Language: English
• Subjects: e基因; 克隆; 快速扩增cDNA末端; 烟酰胺腺嘌呤二核苷酸; 环化酶; 甘薯品种; 番茄红素; 类胡萝卜素含量
• ISSN: 2095-3119; 2352-3425

This paper reported firstly successful cloning of lycopene ε-cyclase （lbLCYe） gene from sweetpotato, lpomoea batatas （L.） Lam. Using rapid amplification of cDNA ends （RACE）, lbLCYe gene was cloned from sweetpotato cv. Nongdafu 14 with high carotenoid content. The 1 805 bp cDNA sequence oflbLCYe gene contained a 1236 bp open reading frame （ORF） encoding a 411 amino acids polypeptide with a molecular weight of 47 kDa and an isoelectric point （pI） of 6.95. IbLCYe protein contained one potential lycopene ε-cyclase domain and one potential FAD （flavinadenine dinucleotide）/NAD（P） （nicotinamide adenine dinucleotide phosphate）-binding domain, indicating that this protein shares the typical characteristics of LCYe proteins. The gDNA oflbLCYe gene was 4 029 bp and deduced to contain 5 introns and 6 exons. Real-time quantitative PCR analysis revealed that the expression level of IbLCYe gene was significantly higher in the storage roots of Nongdafu 14 than those in the leaves and stems. Transgenic tobacco （cv. Wisconsin 38） expressing [bLCYe gene accumulated significantly more ~-carotene compared to the untransformed control plants. These results showed that lbLCYe gene has an important function for the accumulation of carotenoids of sweetpotato.