Derivation of androgenetic embryonic stem cells from m-carboxycinnamic acid bishydroxamide （CB HA） treated androgenetic embryos

• Published in: 中国科学通报：英文版 2013 (23), p.2862-2868
• Main Author: SHUAI Ling FENG ChunJing ZHANG HaiJiang GU Qi JIA YunDan WANG Liu ZHAO Xiao-Yang LIU ZhongHua ZHOU Qi
• Format: Article
• Language: English
• Subjects: CAES; ES细胞系; 印记基因; 治疗; 胚胎干细胞; 表达模式; 雄性
• ISSN: 1001-6538; 1861-9541

The androgenetic embyronic stem （aES） cells are useful models in studying the effects of imprinted genes on pluripotency maintaining and embryo development. The expression patterns of imprinted genes are significantly different between uniparental derived aES cells and zygote-derived embryonic stem （ES） cells, therefore, the imprinting related cell pluripotency needs further exploitation. Several approaches have been applied in generation of androgenetic embryos and derivation of aES cell lines. Here, we describe a method to generate androgenetic embryos by injecting two mature sperms into one enucleated oocyte. Then these androgenetic embryos were treated with a histone deacetylase inhibitor： m-carboxycinnamic acid bishydroxamide （CBHA）. Further, aES cell lines were successfully derived from these treated androgenetic embryos at blastocyst stage. The CBHA could improve not only the quality of androgenetic embryos, but also the efficiencies of aES （CaES） cells derivation and chimeric mice generation. The imprinted gene expression pattern in the CBHA treated embryo-derived aES （CaES） cells was also highly similar to that of zygote-derived ES cells.