Layer by layer surface engineering of poly （lactide-co-glycolide） nanoparticles： A versatile tool for nanoparticle engineering for targeted drug delivery

• Published in: 中国科学：化学英文版 2013 (8), p.1029-1039
• Main Author: ROMERO Gabriela MURRAY Richard A SANZ David MOYA Sergio E
• Format: Article
• Language: English
• Subjects: 乙醇酸; 乳酸; 多功能; 纳米层; 纳米工程; 蛋白质相互作用; 表面层; 靶向给药
• ISSN: 1674-7291; 1869-1870

Recent work regarding the Layer by Layer （LbL） engineering of poly（lactide-co-glycolide） nanoparticles （PLGA NPs） is reviewed here. The LbL engineering of PLGA NPs is applied as a means of generating advanced drug delivery devices with tailored recognition, protection, cargo and release properties. LbL in combination with covalent chemistry is used to attach PEG and folic acid to control cell uptake and direct it towards cancer cells. LbL coatings composed of chitosan and alginate show low protein interactions and can be used as an alternative to Pegylation. The assembly on top of LbL coatings of lipid layers composed of variable percentages of 1,2-dioleoyl-sn-glycero-3-choline （DOPC） and 1,2-dioleoyl-sn-glycero-3-phospho- L-serine （DOPS） increases NP uptake and directs the NPs towards the endoplasmic reticulum. The antibody anti-TNF-ct is encapsulated forming a complex with alginate that is assembled LbL on top of PLGA NPs. The antibody is released in cell culture following first order kinetics. The release kinetics of encapsulated molecules inside PLGA NPs are studied when the PLGA NPs are coated via LbL with different polyelectrolytes. The intracellular release of encapsulated Doxorubicin is studied in the HepG2 cell line by means of Fluorescence Lifetime Imaging.