Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-p-CD as a chiral selector

A simple, sensitive and selective method of high-performance liquid chromatography （HPLC） has been successfully developed for separation of bavachinin enantiomers in Fructus Psoraleae and rat plasma. The separation and detection conditions of HPLC were optimized. Chiral bavachinin were separated wit...

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Bibliographic Details
Published in:药物分析学报：英文版 2013, Vol.3 (5), p.349-353
Main Author: Jing-Jing Liu Juan Zhang Zi-Lin Chen
Format: Article
Language:English
Subjects:
大鼠
手性分离
手性选择
液体色谱法
液相色谱仪
血浆分离
补骨脂
高效液相色谱法
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Summary:A simple, sensitive and selective method of high-performance liquid chromatography （HPLC） has been successfully developed for separation of bavachinin enantiomers in Fructus Psoraleae and rat plasma. The separation and detection conditions of HPLC were optimized. Chiral bavachinin were separated with the mobile phase of methanol and water （70：30, v/v） at a flow rate of 1.0 mL/min. The linear ranges were in the range of 20-1000 μg/mL. The detection limits were tested as 4 ng/mL and 6 ng/mL for （＋）-bavachinin and （-）-bavachinin, respectively. The method has been applied to analyze chiral bavachinin in rat plasma. HPLC-MS method was used to test the accuracy.
ISSN:2095-1779
2214-0883