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Effects of Fengbaisan (丰白散) on the Expression of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease

Objective: To observe effects of Fengbaisan (丰白散, FBS) on the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in lung tissue of rats with chronic obstructive pulmonary disease (COPD) and to investigate the preventive and therapeutic mechanisms of...

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Published in:中国结合医学杂志:英文版 2014 (3), p.224-231
Main Author: WANG Yu SU Nan-xiang CHEN Ze-qi WANG Zhe ZHANG Si-fang
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description Objective: To observe effects of Fengbaisan (丰白散, FBS) on the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in lung tissue of rats with chronic obstructive pulmonary disease (COPD) and to investigate the preventive and therapeutic mechanisms of FBS. Methods: The COPD rat model was established by cigarette smoke exposure and lipopolysaccharide (LPS) intra-tracheal dripping. The histopathological changes of lung tissue was observed via hematoxylin/eosin staining. The expression of MMP-9 and TIMP-1 in lung tissue was measured by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. Results: The typical histopathological changes of COPD were displayed in the model group, Ambroxol Hydrochloride group and FBS group, and the pathological lesions in the FBS group were less than those in the model group. The expression of MMP-9 and TIMP-1 in the model group increased significantly compared with those in the normal group (P〈0.05). After treatment for successive 28 days, the expression of MMP-9 and TIMP-1 in the FBS group decreased remarkably as compared with the model group (P〈0.05). Conclusions: FBS can regulate MMP-9/TIMP-1 imbalance to prevent airway and lung parenchyma remodeling process via reducing the expression of MMP-9 and TIMP-1 in the lung tissue of COPD rats, and this may be a possible therapeutic mechanism of FBS on COPD. KEYWORDS chronic obstructive pulmonary disease, Fengbaisan, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, airway remodeling, Chinese medicine
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Methods: The COPD rat model was established by cigarette smoke exposure and lipopolysaccharide (LPS) intra-tracheal dripping. The histopathological changes of lung tissue was observed via hematoxylin/eosin staining. The expression of MMP-9 and TIMP-1 in lung tissue was measured by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. Results: The typical histopathological changes of COPD were displayed in the model group, Ambroxol Hydrochloride group and FBS group, and the pathological lesions in the FBS group were less than those in the model group. The expression of MMP-9 and TIMP-1 in the model group increased significantly compared with those in the normal group (P〈0.05). After treatment for successive 28 days, the expression of MMP-9 and TIMP-1 in the FBS group decreased remarkably as compared with the model group (P〈0.05). Conclusions: FBS can regulate MMP-9/TIMP-1 imbalance to prevent airway and lung parenchyma remodeling process via reducing the expression of MMP-9 and TIMP-1 in the lung tissue of COPD rats, and this may be a possible therapeutic mechanism of FBS on COPD. KEYWORDS chronic obstructive pulmonary disease, Fengbaisan, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, airway remodeling, Chinese medicine</description><identifier>ISSN: 1672-0415</identifier><identifier>EISSN: 1993-0402</identifier><language>eng</language><subject>MMP-9 ; TIMP-1 ; 基质金属蛋白酶-9 ; 大鼠模型 ; 慢性阻塞性肺病 ; 肺组织 ; 蛋白酶抑制剂 ; 逆转录聚合酶链反应</subject><ispartof>中国结合医学杂志:英文版, 2014 (3), p.224-231</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86437A/86437A.jpg</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids></links><search><creatorcontrib>WANG Yu SU Nan-xiang CHEN Ze-qi WANG Zhe ZHANG Si-fang</creatorcontrib><title>Effects of Fengbaisan (丰白散) on the Expression of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease</title><title>中国结合医学杂志:英文版</title><addtitle>Chinese Journal of Integrative Medicine</addtitle><description>Objective: To observe effects of Fengbaisan (丰白散, FBS) on the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in lung tissue of rats with chronic obstructive pulmonary disease (COPD) and to investigate the preventive and therapeutic mechanisms of FBS. Methods: The COPD rat model was established by cigarette smoke exposure and lipopolysaccharide (LPS) intra-tracheal dripping. The histopathological changes of lung tissue was observed via hematoxylin/eosin staining. The expression of MMP-9 and TIMP-1 in lung tissue was measured by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. Results: The typical histopathological changes of COPD were displayed in the model group, Ambroxol Hydrochloride group and FBS group, and the pathological lesions in the FBS group were less than those in the model group. The expression of MMP-9 and TIMP-1 in the model group increased significantly compared with those in the normal group (P〈0.05). After treatment for successive 28 days, the expression of MMP-9 and TIMP-1 in the FBS group decreased remarkably as compared with the model group (P〈0.05). Conclusions: FBS can regulate MMP-9/TIMP-1 imbalance to prevent airway and lung parenchyma remodeling process via reducing the expression of MMP-9 and TIMP-1 in the lung tissue of COPD rats, and this may be a possible therapeutic mechanism of FBS on COPD. KEYWORDS chronic obstructive pulmonary disease, Fengbaisan, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, airway remodeling, Chinese medicine</description><subject>MMP-9</subject><subject>TIMP-1</subject><subject>基质金属蛋白酶-9</subject><subject>大鼠模型</subject><subject>慢性阻塞性肺病</subject><subject>肺组织</subject><subject>蛋白酶抑制剂</subject><subject>逆转录聚合酶链反应</subject><issn>1672-0415</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNzj1Ow0AQBWALgUT4ucNwAEtObMdxHRyBRARC6aO1GduDnFmzs4ZwAzgABTUXoKQIFVdBUOYKrBBUNFTzRvreaDa8Xj9NQz-IgsGmy8Nk4HI_3vZ2RC6DIE6GQdzz3rKyxMIK6BImyFWuSBTDenX3_vL8-fj68fC0Xt2DZrA1QrZsDYqQW52fKmtoCVO0qml0a7RFYiXop6D4AmYk0iEcc005WW2-K39sH4jhpOPq1zt1rtxDN2RrGNdGMxVwmos1XWHpGuGsaxaalbmFQxJ0N_a8rVI1gvs_c9c7mGSz8ZFf1JqrK-Jq3hpauMY8Go3iNIyS8D_mC0wpbuc</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>WANG Yu SU Nan-xiang CHEN Ze-qi WANG Zhe ZHANG Si-fang</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope></search><sort><creationdate>2014</creationdate><title>Effects of Fengbaisan (丰白散) on the Expression of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease</title><author>WANG Yu SU Nan-xiang CHEN Ze-qi WANG Zhe ZHANG Si-fang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_488593473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>MMP-9</topic><topic>TIMP-1</topic><topic>基质金属蛋白酶-9</topic><topic>大鼠模型</topic><topic>慢性阻塞性肺病</topic><topic>肺组织</topic><topic>蛋白酶抑制剂</topic><topic>逆转录聚合酶链反应</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WANG Yu SU Nan-xiang CHEN Ze-qi WANG Zhe ZHANG Si-fang</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>中国结合医学杂志:英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WANG Yu SU Nan-xiang CHEN Ze-qi WANG Zhe ZHANG Si-fang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Fengbaisan (丰白散) on the Expression of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease</atitle><jtitle>中国结合医学杂志:英文版</jtitle><addtitle>Chinese Journal of Integrative Medicine</addtitle><date>2014</date><risdate>2014</risdate><issue>3</issue><spage>224</spage><epage>231</epage><pages>224-231</pages><issn>1672-0415</issn><eissn>1993-0402</eissn><abstract>Objective: To observe effects of Fengbaisan (丰白散, FBS) on the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in lung tissue of rats with chronic obstructive pulmonary disease (COPD) and to investigate the preventive and therapeutic mechanisms of FBS. Methods: The COPD rat model was established by cigarette smoke exposure and lipopolysaccharide (LPS) intra-tracheal dripping. The histopathological changes of lung tissue was observed via hematoxylin/eosin staining. The expression of MMP-9 and TIMP-1 in lung tissue was measured by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. Results: The typical histopathological changes of COPD were displayed in the model group, Ambroxol Hydrochloride group and FBS group, and the pathological lesions in the FBS group were less than those in the model group. The expression of MMP-9 and TIMP-1 in the model group increased significantly compared with those in the normal group (P〈0.05). After treatment for successive 28 days, the expression of MMP-9 and TIMP-1 in the FBS group decreased remarkably as compared with the model group (P〈0.05). Conclusions: FBS can regulate MMP-9/TIMP-1 imbalance to prevent airway and lung parenchyma remodeling process via reducing the expression of MMP-9 and TIMP-1 in the lung tissue of COPD rats, and this may be a possible therapeutic mechanism of FBS on COPD. KEYWORDS chronic obstructive pulmonary disease, Fengbaisan, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, airway remodeling, Chinese medicine</abstract></addata></record>
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language eng
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source Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List
subjects MMP-9
TIMP-1
基质金属蛋白酶-9
大鼠模型
慢性阻塞性肺病
肺组织
蛋白酶抑制剂
逆转录聚合酶链反应
title Effects of Fengbaisan (丰白散) on the Expression of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease
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