Major Gene Identification and Quantitative Trait Locus Mapping for Yield- Related Traits in Upland Cotton （Gossypium hirsutum L.）

• Published in: 农业科学学报：英文版 2014 (2), p.299-309
• Main Author: XIA Zhe ZHANG Xin LIU Yang-yang JIA Zi-fang ZHAO Hai-hong LI Cheng-qi WANG Qing-lian
• Format: Article
• Language: English
• Subjects: QTL定位; 主基因＋多基因; 产量相关性状; 基因鉴定; 数量性状位点; 映射; 棉花; 陆地棉
• ISSN: 2095-3119; 2352-3425

Segregation analysis of the mixed genetic model of major gene plus polygene was used to identify the major genes for cotton yield-related traits using six generations P1, P2, F1, B1, B2, and F2 generated from the cross of Baimian 1 x TM-1. In addition to boll size and seed index, the major genes for the other five traits were detected： one each for seed yield, lint percentage, boll number, lint index; and two for lint yield. Quantitative trait locus/loci （QTL） mapping was performed in the F2 and F2：3 populations of above cross through molecular marker technology, and a total of 50 QTL （26 suggestive and 24 significant） for yield-related traits were detected. Four common QTL were discovered： qLP-3b（F2）/qLP-3（F2：3） and qLP-19b （F2）/qLP-19（F2：3） for lint percentage, qBN-17（F2）/qBN-17（F2：3） for boll number, and qBS-26b（F2）/qBS-26（F2：3） for boll size. Especially, qLP- 3b（Fz）/qLP-3（F2：3）, not only had LOD scores 〉3 but also exceeded the permutation threshold （5.13 and 5.29, respectively）, correspondingly explaining 23.47 and 29.55% of phenotypic variation. This QTL should be considered preferentially in marker assisted selection （MAS）. Segregation analysis and QTL mapping could mutually complement and verify, which provides a theoretical basis for genetic improvement of cotton yield-related traits by using major genes （QTL）.