Occurrence and characterization of toxigenic Bacillus cereus in food and infant feces

Objective: To investigate the true incidence of Bacillus cereus(B. cereus) in food and children diarrhea cases. Methods: A total of 110 samples of various dairy products such as raw milk, long life pasteurized milk, yoghurt and infant powdered milk formulas, raw rice, and feces were examined for the...

Full description

Saved in:
Bibliographic Details
Published in:亚太热带生物医学杂志：英文版 2015 (7), p.510-514
Main Author: Sameer Rushdi Organji Hussein Hasan Abulreesh Khaled Elbanna Gamal Ebrahim Haridy Osman Manal Khider
Format: Article
Language:English
Subjects:
Bacillus
cereus
Diarrhea
Enterotoxin
Food
formula
Infant
licheniformis
milk
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 514
container_issue 7
container_start_page 510
container_title 亚太热带生物医学杂志：英文版
container_volume
creator Sameer Rushdi Organji Hussein Hasan Abulreesh Khaled Elbanna Gamal Ebrahim Haridy Osman Manal Khider
description Objective: To investigate the true incidence of Bacillus cereus(B. cereus) in food and children diarrhea cases. Methods: A total of 110 samples of various dairy products such as raw milk, long life pasteurized milk, yoghurt and infant powdered milk formulas, raw rice, and feces were examined for the presence of B. cereus by selective plating on mannitol-egg-yolk-polymyxin agar. Confirmation of B. cereus was carried out by biochemical tests and PCR. Identification of non-B. cereus isolates was carried out by 16 S r DNA sequencing. Antimicrobial susceptibility was done by disk diffusion method.Results: Overall 35 samples(31.8%, n = 110) yielded Bacillus-like growth. Of which 19 samples(54.28%) were positive for B. cereus. All isolates were positive for enterotoxin production. No psychrotolerant B. cereus strains were detected in all samples. All B. cereus isolates were resistant to penicillin G, but susceptible to vancomycin, erythromycin and clindamycin. Conclusions: The results of this study confirm the importance of including B. cereus in disease control and prevention programs, as well as in routine clinical and food quality control laboratories in both Saudi Arabia and Egypt.
format article
fullrecord <record><control><sourceid>chongqing</sourceid><recordid>TN_cdi_chongqing_primary_65808466504849534855484851</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>65808466504849534855484851</cqvip_id><sourcerecordid>65808466504849534855484851</sourcerecordid><originalsourceid>FETCH-chongqing_primary_658084665048495348554848513</originalsourceid><addsrcrecordid>eNqdi0FqwzAQAEVIISHxH_QBgyxLQr62NPTWS3oOYr2ytzirVHIgyetrSl7QucxcZiW22npfd1rr9dJaN3XjumYjqlK-1YJTWluzFV-fANeckQFl4F7CGHKAGTM9wkyJZYpyTjcakAnkawCapmuRgBkXEcuYUv93EsfAs4wIWPbiJYapYPX0TrSH9-PbRw1j4uGHeDhdMp1Dvp-c9cob56wy3nS2Nd7apbxt2v9dv_VgSU4</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Occurrence and characterization of toxigenic Bacillus cereus in food and infant feces</title><source>Elsevier ScienceDirect Journals</source><creator>Sameer Rushdi Organji Hussein Hasan Abulreesh Khaled Elbanna Gamal Ebrahim Haridy Osman Manal Khider</creator><creatorcontrib>Sameer Rushdi Organji Hussein Hasan Abulreesh Khaled Elbanna Gamal Ebrahim Haridy Osman Manal Khider</creatorcontrib><description>Objective: To investigate the true incidence of Bacillus cereus(B. cereus) in food and children diarrhea cases. Methods: A total of 110 samples of various dairy products such as raw milk, long life pasteurized milk, yoghurt and infant powdered milk formulas, raw rice, and feces were examined for the presence of B. cereus by selective plating on mannitol-egg-yolk-polymyxin agar. Confirmation of B. cereus was carried out by biochemical tests and PCR. Identification of non-B. cereus isolates was carried out by 16 S r DNA sequencing. Antimicrobial susceptibility was done by disk diffusion method.Results: Overall 35 samples(31.8%, n = 110) yielded Bacillus-like growth. Of which 19 samples(54.28%) were positive for B. cereus. All isolates were positive for enterotoxin production. No psychrotolerant B. cereus strains were detected in all samples. All B. cereus isolates were resistant to penicillin G, but susceptible to vancomycin, erythromycin and clindamycin. Conclusions: The results of this study confirm the importance of including B. cereus in disease control and prevention programs, as well as in routine clinical and food quality control laboratories in both Saudi Arabia and Egypt.</description><identifier>ISSN: 2221-1691</identifier><identifier>EISSN: 2588-9222</identifier><language>eng</language><subject>Bacillus ; cereus ; Diarrhea ; Enterotoxin ; Food ; formula ; Infant ; licheniformis ; milk</subject><ispartof>亚太热带生物医学杂志：英文版, 2015 (7), p.510-514</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/71871X/71871X.jpg</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids></links><search><creatorcontrib>Sameer Rushdi Organji Hussein Hasan Abulreesh Khaled Elbanna Gamal Ebrahim Haridy Osman Manal Khider</creatorcontrib><title>Occurrence and characterization of toxigenic Bacillus cereus in food and infant feces</title><title>亚太热带生物医学杂志：英文版</title><addtitle>亚太热带生物医学杂志(英文版)</addtitle><description>Objective: To investigate the true incidence of Bacillus cereus(B. cereus) in food and children diarrhea cases. Methods: A total of 110 samples of various dairy products such as raw milk, long life pasteurized milk, yoghurt and infant powdered milk formulas, raw rice, and feces were examined for the presence of B. cereus by selective plating on mannitol-egg-yolk-polymyxin agar. Confirmation of B. cereus was carried out by biochemical tests and PCR. Identification of non-B. cereus isolates was carried out by 16 S r DNA sequencing. Antimicrobial susceptibility was done by disk diffusion method.Results: Overall 35 samples(31.8%, n = 110) yielded Bacillus-like growth. Of which 19 samples(54.28%) were positive for B. cereus. All isolates were positive for enterotoxin production. No psychrotolerant B. cereus strains were detected in all samples. All B. cereus isolates were resistant to penicillin G, but susceptible to vancomycin, erythromycin and clindamycin. Conclusions: The results of this study confirm the importance of including B. cereus in disease control and prevention programs, as well as in routine clinical and food quality control laboratories in both Saudi Arabia and Egypt.</description><subject>Bacillus</subject><subject>cereus</subject><subject>Diarrhea</subject><subject>Enterotoxin</subject><subject>Food</subject><subject>formula</subject><subject>Infant</subject><subject>licheniformis</subject><subject>milk</subject><issn>2221-1691</issn><issn>2588-9222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqdi0FqwzAQAEVIISHxH_QBgyxLQr62NPTWS3oOYr2ytzirVHIgyetrSl7QucxcZiW22npfd1rr9dJaN3XjumYjqlK-1YJTWluzFV-fANeckQFl4F7CGHKAGTM9wkyJZYpyTjcakAnkawCapmuRgBkXEcuYUv93EsfAs4wIWPbiJYapYPX0TrSH9-PbRw1j4uGHeDhdMp1Dvp-c9cob56wy3nS2Nd7apbxt2v9dv_VgSU4</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Sameer Rushdi Organji Hussein Hasan Abulreesh Khaled Elbanna Gamal Ebrahim Haridy Osman Manal Khider</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope></search><sort><creationdate>2015</creationdate><title>Occurrence and characterization of toxigenic Bacillus cereus in food and infant feces</title><author>Sameer Rushdi Organji Hussein Hasan Abulreesh Khaled Elbanna Gamal Ebrahim Haridy Osman Manal Khider</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_658084665048495348554848513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bacillus</topic><topic>cereus</topic><topic>Diarrhea</topic><topic>Enterotoxin</topic><topic>Food</topic><topic>formula</topic><topic>Infant</topic><topic>licheniformis</topic><topic>milk</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sameer Rushdi Organji Hussein Hasan Abulreesh Khaled Elbanna Gamal Ebrahim Haridy Osman Manal Khider</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>亚太热带生物医学杂志：英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sameer Rushdi Organji Hussein Hasan Abulreesh Khaled Elbanna Gamal Ebrahim Haridy Osman Manal Khider</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence and characterization of toxigenic Bacillus cereus in food and infant feces</atitle><jtitle>亚太热带生物医学杂志：英文版</jtitle><addtitle>亚太热带生物医学杂志(英文版)</addtitle><date>2015</date><risdate>2015</risdate><issue>7</issue><spage>510</spage><epage>514</epage><pages>510-514</pages><issn>2221-1691</issn><eissn>2588-9222</eissn><abstract>Objective: To investigate the true incidence of Bacillus cereus(B. cereus) in food and children diarrhea cases. Methods: A total of 110 samples of various dairy products such as raw milk, long life pasteurized milk, yoghurt and infant powdered milk formulas, raw rice, and feces were examined for the presence of B. cereus by selective plating on mannitol-egg-yolk-polymyxin agar. Confirmation of B. cereus was carried out by biochemical tests and PCR. Identification of non-B. cereus isolates was carried out by 16 S r DNA sequencing. Antimicrobial susceptibility was done by disk diffusion method.Results: Overall 35 samples(31.8%, n = 110) yielded Bacillus-like growth. Of which 19 samples(54.28%) were positive for B. cereus. All isolates were positive for enterotoxin production. No psychrotolerant B. cereus strains were detected in all samples. All B. cereus isolates were resistant to penicillin G, but susceptible to vancomycin, erythromycin and clindamycin. Conclusions: The results of this study confirm the importance of including B. cereus in disease control and prevention programs, as well as in routine clinical and food quality control laboratories in both Saudi Arabia and Egypt.</abstract></addata></record>
fulltext fulltext
identifier ISSN: 2221-1691
ispartof 亚太热带生物医学杂志：英文版, 2015 (7), p.510-514
issn 2221-1691
2588-9222
language eng
recordid cdi_chongqing_primary_65808466504849534855484851
source Elsevier ScienceDirect Journals
subjects Bacillus
cereus
Diarrhea
Enterotoxin
Food
formula
Infant
licheniformis
milk
title Occurrence and characterization of toxigenic Bacillus cereus in food and infant feces
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T23%3A00%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-chongqing&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Occurrence%20and%20characterization%20of%20toxigenic%20Bacillus%20cereus%20in%20food%20and%20infant%20feces&rft.jtitle=%E4%BA%9A%E5%A4%AA%E7%83%AD%E5%B8%A6%E7%94%9F%E7%89%A9%E5%8C%BB%E5%AD%A6%E6%9D%82%E5%BF%97%EF%BC%9A%E8%8B%B1%E6%96%87%E7%89%88&rft.au=Sameer%20Rushdi%20Organji%20Hussein%20Hasan%20Abulreesh%20Khaled%20Elbanna%20Gamal%20Ebrahim%20Haridy%20Osman%20Manal%20Khider&rft.date=2015&rft.issue=7&rft.spage=510&rft.epage=514&rft.pages=510-514&rft.issn=2221-1691&rft.eissn=2588-9222&rft_id=info:doi/&rft_dat=%3Cchongqing%3E65808466504849534855484851%3C/chongqing%3E%3Cgrp_id%3Ecdi_FETCH-chongqing_primary_658084665048495348554848513%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_cqvip_id=65808466504849534855484851&rfr_iscdi=true