A Novel Fluorescence Probe 9-（4-（1,2-diamine）benzeneN 1-phenyl）acridine for Nitric Oxide Determination

A novel fluorescent probe 9-（4-（1,2-diamine）benzene-N1-phenyl）acridine（DABPA） was synthesized for the detection of nitric oxide（NO） and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelectron transfer mechanism, the fl uorescence intensities of DABPA were investigated with the d...

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Bibliographic Details
Published in:武汉理工大学学报：材料科学英文版 2014 (4), p.848-853
Main Author: DING Liyun YUAN Fang HUANG Lanfen HUANG Jun LIU Xiaofang LIANG Bing
Format: Article
Language:English
Subjects:
L-精氨酸
PC12细胞
一氧化氮
二胺
最佳实验条件
氮测定
苯基
荧光探针
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Summary:A novel fluorescent probe 9-（4-（1,2-diamine）benzene-N1-phenyl）acridine（DABPA） was synthesized for the detection of nitric oxide（NO） and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelectron transfer mechanism, the fl uorescence intensities of DABPA were investigated with the different concentrations of NO. Under the optimal experimental conditions, the fl uorescence intensity of DABPA had a good linear relationship（R2=0.9977） with NO concentration in the range from 1×10-7 to 1.5×10-6 mol/L with a detection limit of 1×10-8 mol/L. The cytotoxicity induced by DABPA was evaluated by the MTT（3-（4,5-dimethylthiazol-2-yl）-2,5diphenyl tetrazolium bromide） assay for biological application. Furthermore, the probe DABPA had also been successfully applied to real-time image NO produced in PC12 cells in the presence of L-arginine.
ISSN:1000-2413
1993-0437