Toxic Dinoflagellate Alexandrium tamarense Induces Oxidative Stress and Apoptosis in Hepatopancreas of Shrimp （Fenneropenaeus chinensis）

• Published in: 中国海洋大学学报：英文版 2014, Vol.13 (6), p.1005-1011
• Main Author: LIANG Zhongxiu LI Jian LI Jitao TAN Zhijun REN Hai ZHAO Fazhen
• Format: Article
• Language: English
• Subjects: MDA含量; 中国对虾; 塔玛亚历山大藻; 氧化应激; 细胞凋亡; 肝胰脏; 诱导效应; 谷胱甘肽S-转移酶
• ISSN: 1672-5182; 1993-5021

This study investigated the inductive effect ofAlexandrium tamarense, a toxic dinoflagellate producing paralytic shell- fish poison, on oxidative stress and apoptosis in hepatopancreas of Chinese shrimp, Fenneropenaeus chinensis. The individuals of E chinensis were exposed to 200 and 1000 cells mL-1 of A. tamarense with their superoxide dismutase （SOD）, glutathione S-transferase （GST） activities, malonyldialdehyde （MDA） concentration, and caspase gene （FcCasp） expression in hepatopancreas determined at 12, 24, 48, 72 and 96 h. In addition, apoptosis in hepatopancreas of E chinensis at 96 h after exposure was determined through terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay. The hepatopancreatic SOD and GST activities of F. chinensis exposed to 1000 cells mL-1 ofA. tamarense showed a bell-shaped response to exposure time. The hepatopancreatic MDA concentration ofF. chinensis exposed to 1000 cellsmL-1 ofA. tamarense increased gradually from 48 to 96h, and such a trend corresponded to the decrease of GST activity. The hepatopancreatic FcCasp transcript abundance of F. chinensis exposed to 1000 cells mL-1 ofA. tamarense was positively and linearly correlated to MDA concentration. Results of TUNEL assay showed that exposure to 1000 cells mL-1 of A. tamarense induced apoptosis in the hepatopancreas of E chinensis. Our study revealed that A. tamarense exposure influenced the antioxidative status ofF. chinensis and caused lipid peroxidation and apoptosis in the hepatopancreas of shrimp.