miR-183 potentially inhibits NF-KB1 expression by directly targeting its 3＇-untranslated region

• Published in: 生物化学与生物物理学报：英文版 2014 (11), p.991-996
• Main Author: Fushen Sha Shuangxing Wu Hui Zhang Xuemin Guo
• Format: Article
• Language: English
• Subjects: miRNA; NF-κB; 信号传导途径; 实验证据; 核因子KB; 转录因子; 非翻译区; 预测程序
• ISSN: 1672-9145; 1745-7270

Nuclear factor-KB （NF-KB） is an important transcription factor. While the NF-KB signaling pathway is modulated by many microRNAs （miRNAs）, very few have been reported to target NF-KB1 gene directly. In this study, we used mul- tiple miRNA target prediction programs to predict miRNAs with putative NF-κB1 3＇-untranslated region （UTR） binding sites, miR-183 was strongly implicated and experimentally validated by reporter assays. The results showed a reduced expression of the NF-KB1 3＇UTR containing luciferase vector by -30%, which was comparable to the reduction by miR-9 （the only known miRNA targeting the NF-κB1 3＇UTR）. Mutagenesis of the miR-183 seed region binding sequence in the NF-κB1 3＇UTR abolished the inhibitory effect of miR-183, as noted by the NF-κB1 3＇UTR- containing reporter. Moreover, similar to miR-9, miR-183 could down-regulate the expression of the reporter driven by NF-KB promoter to some degree, suggesting that miR- 183 might negatively regulate the endogenous NF-KB1. Overall, our data provide computational and experimental evidence that NF-κB1 is a potential target of miR-183.