Pharmacokinetic investigation on nteraction between hydrophilic lithospermic acid B and lipophilic tanshinone IIA in rats：an experimental study

• Published in: 中医杂志：英文版 2015-04 (2), p.206-210
• Main Author: Wang Xiuli Gao Wei Sun Mao
• Format: Article
• Language: English
• Subjects: 丹参酮ⅡA; 丹酚酸B; 亲水性; 亲脂性; 动力学相互作用; 大鼠; 平均滞留时间; 药代动力学
• ISSN: 0255-2922

OBJECTIVE：To elucidate the interaction between hydrophilic lithospermic acid B and lipophilic tanshinone Ⅱ A in rats.METHODS：A reliable high-performance liquid chromatography method was adopted for simultaneous determination of lithospermic acid B and tanshinone Ⅱ A in rat plasma,through which the pharmacokinetic interaction between lithospermic acid B and tanshinone Ⅱ A by intravenous injection was investigated.RESULTS：The simultaneous intravenous injection of tanshinone Ⅱ A and lithospermic acid B significantly altered the pharmacokinetic parameters of both compounds when compared with the individual intravenous administration of each compound.The area under the concentration-time curve of tanshinone Ⅱ A and lithospermic acid B increased by 18.35 and 59.31%,respectively.The mean retention time of tanshinone Ⅱ A and lithospermic acid B increased,respectively,from 9.3 to 32.8 h and20.2 to 49.1 h.The concomitant use of tanshinoneⅡ A magnified the volume of distribution at steady state（V_（ss）） and time for the drug in the plasma to reduce the highest concentration by half（t_（1/2）） of lithospermic acid B,while at the same time the V_（ss） and t_（1/2）of tanshinone Ⅱ A changed significantly in the presence of lithospermic acid B.CONCLUSION：Lithospermic acid B and tanshinone D A interact with each other following simultaneous intravenous injection in rats and this observation may expand the clinical use of Danshen（Radix Salviae Miltiorrhizae）.