Effects of different Chinese herbal prescriptions on cytokines in autoimmune prostatitis rats

• Published in: 中医杂志：英文版 2015-04 (2), p.211-217
• Main Author: Song Guohong Zhang Qiumei Pang Baozhen He Lijuan Saimaiti Julaiti Tulahong Aisikeer Gao Xuan You Lina Wuxiuer Reyihan Zhou Wentao Pang Qingyang
• Format: Article
• Language: English
• Subjects: dose; IL-6; IL-8; Wistar大鼠; 中药方剂; 慢性前列腺炎; 细胞因子; 自身免疫性
• ISSN: 0255-2922
• DOI: 10.1016/S0254-6272(15)30030-3

OBJECTIVE：To observe and compare the effects of Chinese herbal prescriptions for promoting blood circulation,clearing heat,removing toxicity,and dispersing stagnated liver-Qi on cytokines in model rats with experimental autoimmune prostatitis（EAP） to provide an experimental basis for the use of Chinese herbal prescriptions in the treatment of chronic prostatitis.METHODS：One-hundred and ten male Wistar rats were randomly divided into 11 groups：blank group;model group;Huoxuehuayu（promoting blood circulation to remove blood stasis） high,middle,and low dose groups;Qingrejiedu（clearing heat and removing toxicity） high,middle,and low dose groups;and Shuganliqi（dispersing stagnated liver-Qi） high,middle,and low dose groups.Except the blank group,rats in all groups were injected subcutaneously in multiple points on days 0 and 30 with prostatic protein extractive solution（60 mg/ml_）,and intraperitoneally injected with diphtheria-pertussis and tetanus vaccine（DPT vaccine） to establish the EAP model.Model rats were administrated high,middle,and low doses of Chinese herbal prescriptions and were sacrificed after 4 weeks.Pathological changes in the prostate gland were observed with HE staining and changes in serum interleukin-6（IL-6）,interleukin-8（IL-8）,and prostaglandin E_2（PGE_2） levels were detected with enzyme-linked immunosorbent assay.RESULTS：Compared with the blank group,serum PGE_2,IL-6,and IL-8 levels in the model group were significantly higher（P 〈 0.05）.Compared with the model group,serum PGE_2,IL-6,and IL-8 levels in the Qingrejiedu low dose and middle dose groups were significantly lower（P 〈 0.05）,with the lower dose having a more obvious effect.Serum PGE_2,IL-6,and IL-8 levels in the Huoxuehuayu high dose group（P 〈 0.05）,IL-6 and IL-8 levels in the Huoxuehuayu middle dose group（P 〈 0.05）,and the IL-8level in the Huoxuehuayu low dose group were significantly lower than those in the model group（P 〈 0.05）.There were significant differences in PGE_2 and IL-6 levels among the different dose groups of Shuganliqi drugs（P 〈 0.05）.Compared with the model group,serum PGE_（2/） IL-6,and IL-8levels in the Shuganliqi high dose group（P 〈0.05） and IL-8 level in the Shuganliqi low dose group were significantly lower（P 〈 0.05）,while the Shuganliqi middle dose group did not change significantly（P 〉 0.05）.CONCLUSION：Therefore,in TCM treatment of autoimmune prostatitis,different treatment methods should select different doses.For prescriptions that clear heat and remove toxic