Cloning and characterization of gene-resistant analogs （RGAs） involved in rust （Puccinia psidii） resistance in Eucalyptus grandis

Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. Most of the disease-resistant genes encode pro- teins with conserved leucine rich repeat and nucleotide binding site domains. In this study, we cloned and char- acterized gene-resis...

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Published in:林业研究：英文版 2015, Vol.26 (3), p.711-718
Main Author: Marcelo Luiz Laia Acelino Couto Alfenas Sergio Herminio Brommonschenkel Shinitiro Oda Eduardo Jose de Melo Inae Marie de Araujo Silva Janalna Fernandes Goncalves Ariadne Marques
Format: Article
Language:English
Subjects:
RGA
克隆
叶锈菌
富含亮氨酸重复序列
巨桉
抗性基因
类似物
野生番石榴
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Summary:Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. Most of the disease-resistant genes encode pro- teins with conserved leucine rich repeat and nucleotide binding site domains. In this study, we cloned and char- acterized gene-resistant analogs （RGAs） from Eucalyptus grandis using degenerate PCR, with primers specifically targeting these two domains. The amplified fragments were cloned into the pGEM-T vector and transformed into Escherichia coli. Among the 90 clones obtained, 13 were sequenced and compared with each other and with previ- ously identified gene-resistant diseases. A BLASTX search in GenBank revealed high similarities among the con- served domains of these cloned genes with RGA genes. Some clones, however, showed no significant similarity with DNA sequences in GenBank. Southern blotting ana- lysis identified several polymorphic RFLP loci between distinct genotypes. However, none of them co-segregated with the Puccinia psidii Winter resistance gene 1 （Ppr1） in a population study.
ISSN:1007-662X
1993-0607