A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana

The newly developed CRISPR （Clustered Regularly Interspaced Short Palindromic Repeats）/Cas （CRISPR-associated） system has emerged as an efficient tool for genome-editing, providing an alternative to clas- sical mutagenesis and transgenic methods to study gene function and improve crop traits. CRISPR...

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Bibliographic Details
Published in:中国科学通报：英文版 2015 (15), p.1332-1347
Main Author: Wenshan Liu Xiaohong Zhu Mingguang Lei Qingyou Xia Jose Ramon Botella Jian-Kang Zhu Yanfei Mao
Format: Article
Language:English
Subjects:
DNA裂解
RNA编辑
介导
协议描述
基因组
拟南芥
程序
转基因载体
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Summary:The newly developed CRISPR （Clustered Regularly Interspaced Short Palindromic Repeats）/Cas （CRISPR-associated） system has emerged as an efficient tool for genome-editing, providing an alternative to clas- sical mutagenesis and transgenic methods to study gene function and improve crop traits. CRISPR/Cas facilitates targeted gene editing through RNA-guided DNA cleavage followed by cellular DNA repair mechanisms that intro- duce sequence changes at the site of cleavage. Here we describe a detailed procedure for our previously developed and highly efficient CRISPR/Cas9 method that allows the generation of heritable-targeted gene mutations and corrections in Arabidopsis. This protocol describes the strategies and steps for the selection of targets, design of single-guide RNA （sgRNA）, vector construction and analysis of transgenic lines. We also offer a method to target two loci simultaneously using vectors containing two different sgRNAs. The principles described in this protocol can be applied to other plant species to generate stably inherited DNA modifications.
ISSN:1001-6538
1861-9541